Anti-ZBTB7A/FBI-1/LRF Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O95365

Core Product Specifications and Parameters

Biological Background: ZBTB7A/FBI-1/LRF Function and Localization

• Alternative names include LRF, FBI1, FBI-1, TIP21, ZBTB7, MNDLFH, ZNF857A, and pokemon.
• Transcription factor that represses the transcription of a wide range of genes involved in cell proliferation and differentiation by directly and specifically binding to the consensus sequence 5'-[GA][CA]GACCCCCCCCC-3'. Related references: PMID:14701838, PMID:17595526, PMID:20812024
• Represses transcription both by regulating the organization of chromatin (recruits HDAC1 to the SMAD4-DNA complex) and through the direct recruitment of transcription factors to gene regulatory regions, as well as by directly interacting with transcription factors like SP1 to prevent their DNA binding. Related references: PMID:12004059, PMID:17595526, PMID:20812024, PMID:25514493
• Functions as an androgen receptor (AR) transcriptional corepressor by recruiting NCOR1 and NCOR2 to the androgen response elements (ARE) on target genes, thereby negatively regulating androgen receptor signaling and androgen-induced cell proliferation. Related references: PMID:20812024
• Involved in the switch between fetal and adult globin expression during erythroid cell maturation. Through its interaction with the NuRD complex, it regulates chromatin at the fetal globin genes to repress their transcription. Related references: PMID:26816381
• Regulates cell proliferation through the transcriptional regulation of genes involved in glycolysis and plays a role in adipogenesis by regulating genes involved in adipocyte differentiation. Related references: PMID:26455326, PMID:14701838
• Plays a role, independently of its transcriptional activity, in double-strand break repair via classical non-homologous end joining (cNHEJ). It is recruited to double-strand break sites on damaged DNA and directly regulates the stability and activity of the DNA-dependent protein kinase complex.
• May modulate the splicing activity of KHDRBS1 toward BCL2L1 in a histone deacetylase-dependent manner, thereby negatively regulating the pro-apoptotic effect of KHDRBS1. Related references: PMID:24514149
• Subcellular localization is predominantly nuclear. Widely expressed in tissues, including thymus, tonsil, and adipose. Up-regulated in a subset of lymphomas, as well as in breast, lung, colon, prostate, and bladder carcinomas. Related references: PMID:9927193, PMID:15662416, PMID:14701838

Experimental Guidance and Technical Tips

• The immunogen was a synthetic peptide corresponding to amino acids 450-550 of human ZBTB7A, so the antibody likely recognizes an epitope within this region. Confirm reactivity with full-length protein in your system.
• For Western blot, the predicted molecular weight is approximately 61 kDa. Validate band specificity using appropriate positive and negative controls (e.g., ZBTB7A overexpressing lysates or cell lines with known expression).
• In immunohistochemistry (IHC-P), consider standard antigen retrieval methods and titrate the antibody to optimize specific staining. Include isotype controls.
• For immunofluorescence (IF/ICC), use a permeabilization step suitable for nuclear proteins and counterstain with DAPI to confirm localization.
• ELISA performance depends on plate coating and detection conditions; it is recommended to perform a checkerboard titration for optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research laboratories, offering one-stop access to high-quality IVD raw materials. This antibody targets ZBTB7A, a transcription factor implicated in cancer and developmental processes, and we can further supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and support rare target raw-material sourcing. Whether you need customized assay development or reliable supply chains, CamelBio bridges the gap from concept to clinic.