Anti-ZBTB6 Rabbit Polyclonal Antibody for WB, ELISA - Q15916

Core Product Specifications and Parameters

Biological Background: ZBTB6 Function and Localization

• Zinc finger and BTB domain-containing protein 6 (ZBTB6), also known as zinc finger protein 482 (ZNF482) or zinc finger protein with interaction domain (ZID), is a member of the BTB-ZF (Broad-Complex, Tramtrack, and Bric-à-brac – zinc finger) family of transcription factors.
• ZBTB6 is believed to play a role in transcriptional regulation through its BTB domain, which mediates protein-protein interactions, and its C2H2-type zinc finger domains, which likely bind DNA.
• The protein is localized to the nucleus, consistent with its putative function as a transcription factor.
• ZBTB6 is widely expressed in human tissues, with the highest levels observed in the brain.
• Post-translational modifications include phosphorylation (as identified by proteomics), which may regulate its activity.
• UniProt Keywords: DNA-binding, Metal-binding, Nucleus, Phosphoprotein, Transcription, Zinc-finger, indicating its classification as a metal-binding, DNA-binding transcriptional regulator.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-220 of human ZBTB6, encompassing the N-terminal region which includes the BTB domain. This design may allow recognition of the native protein in applications such as WB and ELISA.
• For WB, a band at approximately 48 kDa is expected (calculated molecular weight ~48.2 kDa). Consider optimizing loading controls and antibody dilutions based on sample type.
• The antibody is reported to cross-react with mouse and rat, which should be validated in the specific model system under study. Perform appropriate controls to confirm specificity.
• For ELISA, the recombinant protein immunogen can be used as a positive control to calibrate assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials and technical support. As a reliable sourcing partner, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and facilitate rare target raw-material procurement. Our expertise includes zinc finger transcription factors like ZBTB6, supporting your development pipeline from concept to clinic.