Anti-ZBTB48 Polyclonal Antibody for WB, ELISA - P10074

Core Product Specifications and Parameters

Biological Background: ZBTB48 Function and Localization

• ZBTB48, also known as HKR3, TZAP, or ZNF855, is a zinc finger and BTB domain-containing protein that functions as a transcription regulator and telomere-binding protein.
• It plays a critical role in telomere length homeostasis by directly binding the double-stranded telomeric repeat 5'-TTAGGG-3', preferentially at telomeres with low shelterin complex occupancy, and initiating telomere trimming to prevent aberrantly long telomeres. Related references: PMID:28082411, PMID:28500257
• As a transcriptional regulator, ZBTB48 binds promoter regions and controls expression of genes such as MTFP1, the tumor suppressor ARF (CDKN2A isoform), and CIITA, a master regulator of MHC class II genes. Related references: PMID:24382891, PMID:28500257, PMID:7969177, PMID:39562739
• ZBTB48 also regulates cellular RNA m6A/m6Am methylation by facilitating recruitment of the RNA demethylase FTO to target mRNAs. Related references: PMID:39300486
• The protein localizes to the nucleus and specifically associates with telomeric chromatin.
• It is expressed in tissues such as adrenal gland and neuroblastoma, suggesting roles in these contexts.
• ZBTB48 contains multiple zinc finger domains and a BTB/POZ domain, characteristic of transcription factors, and is subject to post-translational modifications including phosphorylation and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to the N-terminal 1–280 amino acids of human ZBTB48 (NP_005332.1), a region that includes the BTB domain and part of the zinc finger domain; consider verifying reactivity with full-length protein.
• For WB applications, test on lysates from human or mouse cells, especially those with reported ZBTB48 expression (e.g., adrenal gland, neuroblastoma).
• ELISA is also indicated; optimize coating antigen and detection conditions for your specific assay format.
• Since cross-reactivity is indicated for human and mouse, sequence alignment may help predict reactivity with other species.

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CamelBio supports diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials. From validated antibody pairs to optimized antibodies for telomere and chromatin research, we streamline your supply chain. Contact us to source ZBTB48 antibodies and related reagents for your next diagnostic or assay development project.