Anti-ZBP1 Rabbit Polyclonal Antibody for WB, ELISA - Q9H171

Core Product Specifications and Parameters

Biological Background: ZBP1 Function and Localization

• ZBP1 (Z-DNA-binding protein 1), also known as DAI or DLM-1, is a key innate immune sensor that recognizes Z-RNA structures produced during viral infections, triggering programmed cell death pathways including necroptosis, apoptosis, and pyroptosis (PANoptosis). Related references: PMID:32200799
• Upon Z-RNA binding, ZBP1 interacts with RIPK3 kinase to initiate MLKL phosphorylation and necroptosis, and also activates caspase-8 and the NLRP3 inflammasome for apoptosis and pyroptosis, respectively.
• Beyond its role in cell death, ZBP1 can restrict viral replication through death-independent transcriptional programs; for instance, during Zika virus infection, it upregulates ACOD1/IRG1 and itaconate production to suppress viral genome replication.
• ZBP1 is localized in both the cytoplasm and nucleus, allowing it to detect Z-RNA in different subcellular compartments.
• Highly expressed in lymphatic tissues (lymph nodes, leukocytes, tonsil, bone marrow, spleen) and to a lesser extent in thymus, lung, and liver. Related references: PMID:11842111
• In herpes simplex virus 1 infection, ZBP1 forms hetero-amyloid structures with the viral protein RIR1/ICP6, which may inhibit host necroptosis and facilitate viral evasion.
• Belongs to a family of Z-DNA/RNA-binding proteins; alternative splicing generates multiple isoforms (predicted).
• Associated keywords: antiviral defense, innate immunity, apoptosis, necrosis, DNA/RNA-binding.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–170 of human ZBP1, covering the N-terminal region that may include Z-DNA-binding domains; consider its impact on detecting endogenous ZBP1 in your application.
• For Western blot, expect a band around 46 kDa; validate with appropriate positive and negative controls in your sample system.
• ELISA can be used for quantifying ZBP1; determine optimal antibody concentration by titration in your specific setup.
• Reported cross-reactivity with mouse and rat ZBP1 should be verified in your experimental context, especially if post-translational modifications differ.
• Given ZBP1's role in PANoptosis, this antibody may aid in studying cell death pathways upon viral infection or other stimuli; consider using pathway-specific inhibitors and controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a seamless, one-stop supply chain for IVD raw materials, from validated antibody pairs to bulk ancillary reagents and rare-target sourcing. This ZBP1 antibody supports critical research into innate immunity and PANoptosis, reflecting our commitment to delivering high-quality tools for immunodiagnostics and beyond. Our integrated services and global sourcing capabilities ensure you have reliable access to the materials you need, from concept to clinic.