Anti-ZAP70 Rabbit Monoclonal Antibody for WB, ELISA - P43403

Core Product Specifications and Parameters

Biological Background: ZAP70 Function and Localization

• Tyrosine-protein kinase ZAP-70 (also known as SRK, Syk-related tyrosine kinase) plays an essential role in adaptive immune response regulation.
• Regulates T-cell motility, adhesion, cytokine expression, and thymocyte development; also contributes to primary B-lymphocyte development and activation.
• Recruited to phosphorylated T-cell receptor (TCR) components CD3E and CD247/CD3Z via ITAM motif at the plasma membrane, leading to relief of autoinhibition (PubMed:7509083). Related references: PMID:7509083
• Phosphorylates key adapter proteins LAT and LCP2, initiating a signaling cascade that promotes T-cell proliferation, differentiation, and lymphokine production.
• Controls cytoskeleton modifications, adhesion, and mobility of T-lymphocytes to ensure correct effector delivery to antigen-presenting cells.
• Required for TCR-CD247/CD3Z internalization and degradation through interaction with E3 ubiquitin-protein ligase CBL and adapters SLA and SLA2, modulating TCR surface expression.
• Localizes to the cytoplasm and cell membrane.
• Primarily expressed in T-cells, natural killer cells, early thymocytes, and pro/pre B-cells.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 520–619 near the C-terminus; consider that epitope may be conserved in isoforms or truncated forms.
• For Western blot, expect a band approximately 70 kDa; validate using T-cell lines (e.g., Jurkat) or mouse lymphoid tissues as positive controls.
• In ELISA, verify specificity against recombinant ZAP70 and avoid cross-reactivity with related Syk kinase; optimize coating and detection conditions.
• Post-translational modifications (phosphorylation, ubiquitination) may affect antibody binding; perform appropriate controls in your experimental system.

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CamelBio serves as a comprehensive IVD raw-material partner, providing diagnostic manufacturers and research labs with ready access to high-quality antibodies like this anti-ZAP70 monoclonal antibody for immune signaling studies. Our portfolio covers validated antibody pairs, bulk ancillary reagents, and rare target sourcing to accelerate T-cell and B-cell research from early development to clinical application. Trust CamelBio to simplify your procurement with a one-stop, concept-to-clinic supply solution.