Anti-YY1AP1 Polyclonal Antibody for WB and ELISA - Q9H869

Core Product Specifications and Parameters

Biological Background: YY1AP1 Function and Localization

• YY1AP1, also known as hepatocellular carcinoma-associated protein 2 (HCCA2) or GRNG, is a ubiquitously expressed protein with very low levels in healthy liver but highly expressed in most liver carcinomas.
• Associates with the INO80 chromatin remodeling complex, which is responsible for transcriptional regulation, DNA repair, and replication.
  Related references: PMID:27939641
• Enhances transcription activation by the transcription factor YY1.
  Related references: PMID:14744866
• Plays a role in cell cycle regulation.
  Related references: PMID:17541814, PMID:27939641
• Subcellularly localizes to the cytoplasm and nucleus, with specific presence in the nucleoplasm and nucleolus.
• Post-translational modifications include phosphorylation, and the protein undergoes alternative splicing.

Experimental Guidance and Technical Tips

• For Western blotting, the predicted molecular weight is approximately 88 kDa. Validate detection in relevant cell or tissue lysates (e.g., liver carcinoma lines such as HepG2 or Huh7) given the high expression in liver carcinomas.
• The immunogen corresponds to the N-terminal amino acids 1-80 of human YY1AP1, which may recognize multiple isoforms arising from alternative splicing.
• For ELISA applications, consider using recombinant YY1AP1 protein as a positive control and optimize coating and detection conditions.
• Perform appropriate controls (e.g., no-primary or isotype controls) to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop sourcing partner for diagnostic manufacturers and research laboratories, providing validated antibody pairs, bulk ancillary reagents, and custom rare-target raw materials to accelerate assay development. This anti-YY1AP1 polyclonal antibody supports chromatin remodeling and liver cancer research, and our dedicated team can assist with additional reagent sourcing or technical consultation to streamline your IVD development pipeline from concept to clinic.