Anti-YY1 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA, ChIP - P25490

Core Product Specifications and Parameters

Biological Background: YY1 Function and Localization

• YY1 is a multifunctional transcription factor that binds to the consensus sequence 5'-CCGCCATNTT-3' near transcription start sites, acting as either an activator or repressor depending on the context. Related references: PMID:15329343, PMID:17721549, PMID:24326773
• In the absence of adenovirus E1A protein, YY1 functions as a repressor, but it switches to an activator in the presence of E1A. It also synergizes with SMAD1/SMAD4 in BMP-mediated cardiac gene expression. Related references: PMID:1655281, PMID:15329343
• YY1 is a proposed core component of the INO80 chromatin remodeling complex, linking it to transcriptional regulation, DNA replication, and DNA repair.
• It participates in DNA repair by binding to Holliday junction recombination intermediates and recruits the PR-DUB complex to specific gene-regulatory regions. Related references: PMID:18026119, PMID:28575647, PMID:20805357
• YY1 localizes predominantly to the nucleus matrix.
• Post-translational modifications such as ADP-ribosylation, phosphorylation, and ubiquitination have been identified, potentially modulating its transcriptional activity.
• The protein contains four C2H2-type zinc finger domains essential for DNA binding.
• Genetic variations in YY1 are associated with intellectual disability and other disease phenotypes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within amino acids 200–300 of human YY1, a region that encompasses part of the zinc finger DNA-binding domain. This design may provide target specificity.
• For Western blotting, a band near 45 kDa is expected. Verify the observed molecular weight in your sample system, as post-translational modifications may cause shifts.
• In immunofluorescence/immunocytochemistry, anticipate nuclear localization. Include appropriate controls to confirm staining pattern.
• For ChIP applications, the epitope accessibility in native chromatin may vary; consider optimization of crosslinking and chromatin shearing conditions.
• ELISA performance can be influenced by the peptide coating format; direct or indirect setups should be evaluated.
• Cross-reactivity with mouse and rat allows multi-species studies, but always validate in the relevant species background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw material procurement, serving diagnostic manufacturers, labs, and research institutes globally. This anti-YY1 monoclonal antibody supports investigations into transcriptional regulation and chromatin biology, and we can further assist with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and sourcing of rare-target raw materials. Let us help you accelerate development from concept to clinic.