Anti-YWHAZ (14-3-3 zeta/delta) Rabbit Polyclonal Antibody for WB, IF, IHC, ELISA - P63104

Core Product Specifications and Parameters

Biological Background: YWHAZ (14-3-3 zeta/delta) Function and Localization

• YWHAZ encodes the 14-3-3 protein zeta/delta, an adapter protein that regulates numerous signaling pathways by binding to phosphoserine/phosphothreonine-containing motifs on partner proteins (PubMed:14578935, PubMed:15071501, PubMed:15644438, PubMed:35662396). Related references: PMID:14578935 PMID:15071501 PMID:15644438 PMID:35662396

• It promotes cytosolic retention and inactivation of transcription factor TFEB by binding to phosphorylated TFEB, modulating autophagy and lysosomal biogenesis (PubMed:35662396). Related references: PMID:35662396

• YWHAZ induces ARHGEF7 activity on RAC1, leading to lamellipodia and membrane ruffle formation, and regulates spine maturation in neurons (PubMed:16959763). Related references: PMID:16959763

• Subcellular localization includes the cytoplasm and melanosome.

• The protein undergoes alternative splicing and post-translational modifications such as phosphorylation and acetylation.

• Mutations in YWHAZ are associated with intellectual disability (Disease variant keyword), and the protein interacts with viral proteins (host-virus interaction).

• Additional aliases include KCIP-1 (Protein Kinase C Inhibitor Protein 1), HEL4, and HEL-S-93.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning residues 146-245 of human YWHAZ (NP_003397.1), a region that is highly conserved in mouse and rat, consistent with reported cross-reactivity.
• For Western blotting, a prominent band at ~28 kDa is expected; consider loading positive control lysates from HEK293, HeLa, or Jurkat cells.
• In immunoprecipitation and immunofluorescence assays, use freshly prepared samples and validate with isotype controls or blocking peptides if available.
• For immunohistochemistry (paraffin-embedded sections), antigen retrieval and optimization of antibody dilution are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-YWHAZ polyclonal antibody supports signaling and adapter protein research and assay development. As your sourcing partner, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement to accelerate your project.