Anti-YWHAH Polyclonal Antibody for WB, IF/ICC, ELISA - Q04917

Core Product Specifications and Parameters

Biological Background: YWHAH Function and Localization

• YWHAH encodes the 14-3-3 protein eta (also known as Protein AS1), a member of the 14-3-3 family of highly conserved regulatory proteins.
• Functions as an adapter protein that mediates signal transduction by binding to phosphoserine- or phosphothreonine-containing motifs on target proteins, thereby modulating their activity, localization, and interactions.
• Directly interacts with and negatively regulates the kinase activity of PDPK1 (3-phosphoinositide-dependent protein kinase 1), impacting downstream signaling cascades.
• Predominantly expressed in the brain, with lower expression observed in other tissues, indicating a specialized role in neural signaling.
• Undergoes post-translational modifications including phosphorylation and acetylation, which may influence its binding capacity and functional regulation.
• The resolved three-dimensional structure (available in PDB) provides insights into the molecular basis of its phosphopeptide-binding specificity and dimer formation.
• The canonical protein has a calculated molecular mass of approximately 28.2 kDa, consistent with the observed band at 28 kDa in western blot experiments.

Experimental Guidance and Technical Tips

• For western blotting, a band around 28 kDa is expected in human, mouse, and rat samples. Consider including brain lysates as a positive control due to high expression.
• For immunofluorescence/immunocytochemistry, fixation and permeabilization steps should be optimized; verify nuclear and cytoplasmic localization patterns.
• ELISA suitability has been reported; ensure appropriate coating antigens and detection conditions are validated for sandwich or direct formats.
• The immunogen is a full-length recombinant protein; thus epitope recognition may be conformation-dependent; denaturing conditions might reduce signal in some applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing partner for IVD raw materials, offering ready-to-use polyclonal and monoclonal antibodies, validated antibody pairs, and ancillary reagents for diagnostic development. For research on 14-3-3 eta and signaling pathways, we provide optimized antibodies and can support bulk supply and custom sourcing needs to accelerate your assay development projects.