Anti-YUCCA1 Rabbit Polyclonal Antibody for WB and ELISA - Q9SZY8

Core Product Specifications and Parameters

Biological Background: YUCCA1 Function and Localization

• YUCCA1 is a probable indole-3-pyruvate monooxygenase, also known as flavin-containing monooxygenase YUCCA1, encoded by the YUC1 gene in Arabidopsis thaliana.
• It plays a critical role in auxin biosynthesis, catalyzing the N-oxidation of tryptamine to form N-hydroxyl tryptamine in vitro.
• YUCCA1 is involved in multiple developmental processes, including embryogenesis, seedling development, and the formation of floral organs and vascular tissues.
• It belongs to a family of redundant YUCCA genes that are collectively responsible for auxin biosynthesis in shoots.
• The protein is expressed in apical meristems, young floral primordia, floral meristems, and at the base of floral organs.
• YUCCA1 is a flavoprotein monooxygenase that uses FAD and NADP as cofactors.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 71-221) of YUCCA1, which may provide good detection sensitivity across plant tissues.
• For Western blotting, a band around 46 kDa is expected; consider optimizing protein extraction from appropriate Arabidopsis tissues.
• In ELISA, the antibody can be used for detection of recombinant or native YUCCA1; validate in your specific experimental system.
• Cross-reactivity is reported for Arabidopsis thaliana; if testing other plant species, verify sequence homology and perform validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing high-quality antibodies like this anti-YUCCA1 polyclonal antibody to support plant developmental biology and auxin research. We supply validated antibody pairs, optimized polyclonal and monoclonal antibodies, and ancillary reagents in bulk, helping diagnostic manufacturers and researchers streamline their workflows from concept to clinic.