Anti-YTHDF3 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - Q7Z739

Core Product Specifications and Parameters

Biological Background: YTHDF3 Function and Localization

• YTHDF3 is a member of the YTH domain-containing family (also known as DF3) that specifically binds N6-methyladenosine (m6A)-modified RNAs, a prevalent internal modification influencing mRNA stability and processing.
  Related references:
  PMID:22575960
  PMID:24284625
  PMID:28106072
  PMID:28106076
  PMID:28281539

• It promotes degradation of m6A-containing mRNAs by interacting with the CCR4-NOT deadenylase complex or PAN3, thus acting as a key regulator of mRNA turnover.
  Related references:
  PMID:32492408

• YTHDF3 functions redundantly with its paralogs YTHDF1 and YTHDF2 in mediating m6A-dependent mRNA decay and cellular differentiation, sharing many target transcripts.
  Related references:
  PMID:28106072
  PMID:28106076
  PMID:32492408

• Upon binding polymethylated mRNAs, YTHDF3 undergoes liquid-liquid phase separation, driving the formation of membraneless organelles such as P-bodies and stress granules, which compartmentalize RNA metabolism.
  Related references:
  PMID:31292544
  PMID:31388144
  PMID:32451507

• The protein localizes predominantly to the cytosol, P-bodies, and stress granules, consistent with its role in cytoplasmic RNA processing; it also exhibits antiviral activity against HIV-1 by incorporating into virions and reducing infectivity through m6A recognition on the viral genome.
  Related references:
  PMID:32053707

• YTHDF3 is subject to post-translational modifications including acetylation and phosphorylation, and its RNA-binding function places it at the intersection of epitranscriptomics and cellular stress responses.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal 275 amino acids of human YTHDF3; consider verifying antibody specificity using full‑length protein controls or knockdown/knockout cell lysates in your application.
• In Western blot, a band near 64 kDa is expected; post‑translational modifications or isoform variations may result in additional bands—validate using appropriate molecular weight markers.
• For IF/ICC and IHC‑P, optimize fixation and permeabilization conditions, as YTHDF3 can localize to cytoplasmic granules; co‑staining with a stress granule marker may serve as a positive control.
• The antibody’s cross‑reactivity with mouse and rat suggests conserved epitope recognition; however, users should confirm reactivity in their specific species and tissue context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive sourcing partner for IVD raw materials. For researchers studying m6A RNA modifications and YTHDF-family proteins, we provide access to validated antibody pairs, bulk ancillary reagents, and rare targets. Whether you need this YTHDF3 polyclonal antibody or customized sourcing for related epitranscriptomics tools, we support your journey from concept to clinic.