Anti-YTHDF2 Rabbit Polyclonal Antibody for WB, IHC, IF - Q9Y5A9

Core Product Specifications and Parameters

Biological Background: YTHDF2 Function and Localization

• YTHDF2 is an N6-methyladenosine (m6A) reader protein that specifically binds m6A-containing RNAs and regulates their stability. Related references: PMID:24284625, PMID:26046440, PMID:26318451
• It promotes degradation of m6A mRNAs via CCR4-NOT deadenylation or ribonuclease P/MRP endoribonucleolytic cleavage, depending on the molecular context. Related references: PMID:27558897, PMID:30930054
• YTHDF paralogs (YTHDF1, YTHDF2, YTHDF3) share target mRNAs and act redundantly in mRNA degradation and cellular differentiation. Related references: PMID:28106072, PMID:32492408
• Subcellularly, YTHDF2 localizes to the cytosol, P-bodies, stress granules, and the nucleus, and undergoes liquid-liquid phase separation to form membraneless compartments. Related references: PMID:31292544, PMID:31388144, PMID:31642031
• Highly expressed in induced pluripotent stem cells (iPSCs) and downregulated during neural differentiation, it inhibits neural specification by promoting degradation of methylated neural-specific mRNAs. Related references: PMID:32169943
• YTHDF2 also regulates circadian hepatic lipid metabolism, innate antiviral immunity, mitotic entry, and spermatogenesis through m6A-dependent mRNA decay. Related references: PMID:30428350, PMID:30559377, PMID:32267835
• During viral infection, YTHDF2 can promote gene expression and replication of SV40 and KSHV by binding m6A-modified viral RNAs. Related references: PMID:29447282, PMID:29659627

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1–200) of human YTHDF2; consider this epitope location when designing multiplex assays or when using truncated protein standards.
• For Western blot, the predicted molecular weight is approximately 62 kDa; include appropriate positive control lysates (e.g., HeLa, HEK293T) and optimize protein loading for clear detection.
• In immunofluorescence (IF/ICC, IF-P), fix and permeabilize cells according to the target's cytosolic and granular localization; validate staining patterns in known positive and negative cell lines.
• For immunohistochemistry on paraffin sections (IHC-P), test antigen retrieval conditions (e.g., citrate buffer pH 6.0 or EDTA pH 9.0) and titrate the antibody to reduce background.
• The antibody cross-reacts with mouse and rat YTHDF2, enabling translational studies in rodent models; confirm sequence homology if absolute specificity is critical.
• Since YTHDF2 binds m6A-modified RNAs, consider treating cells with methylation inhibitors or RNase when assessing signal specificity in RNA-dependent contexts.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop IVD raw-material sourcing partner, offering diagnostic manufacturers and researchers access to high-quality antibodies, antigens, and ancillary reagents from concept to clinic. This YTHDF2 polyclonal antibody supports epitranscriptomics and RNA biology research, serving as a robust tool for biomarker discovery and assay development. Beyond individual reagents, CamelBio provides validated antibody pairs, bulk monoclonal/polyclonal antibodies, and custom sourcing for rare targets, ensuring seamless integration into your IVD workflow.