Anti-YTHDF2 (KO Validated) Monoclonal Antibody for WB, IP, IF/ICC, ELISA - Q9Y5A9

Core Product Specifications and Parameters

Biological Background: YTHDF2 Function and Localization

• YTHDF2 specifically binds N6-methyladenosine (m6A)-containing RNAs and regulates their stability by promoting degradation through interactions with the CCR4-NOT deadenylase complex and the ribonuclease P/MRP complex. Related references: PMID:24284625 PMID:26046440 PMID:26318451 PMID:27558897
• The YTHDF paralogs (YTHDF1, YTHDF2 and YTHDF3) share m6A-containing mRNA targets and act redundantly to mediate mRNA degradation and cellular differentiation. Related references: PMID:28106072 PMID:32492408
• Subcellularly, YTHDF2 localizes to the cytosol, P-bodies, stress granules, and can translocate to the nucleus under certain conditions.
• YTHDF2 promotes formation of phase-separated membraneless compartments, such as P-bodies and stress granules, by undergoing liquid-liquid phase separation upon binding to mRNAs with multiple m6A residues. Related references: PMID:31292544 PMID:31388144 PMID:31642031
• It is required for hematopoietic stem cell specification by promoting degradation of m6A-containing mRNAs. Related references: PMID:30065315
• YTHDF2 regulates neural development by inhibiting neural specification through m6A-dependent degradation of neural-specific mRNAs. Related references: PMID:32169943
• It regulates the innate immune response by binding m6A-containing IFNB transcripts and promoting their degradation, thereby inhibiting type I interferon production. Related references: PMID:30559377
• During viral infection, YTHDF2 binds m6A-modified viral RNAs to promote viral gene expression and replication, as observed in SV40 and Kaposi's sarcoma-associated herpesvirus (KSHV). Related references: PMID:29447282 PMID:29659627

Experimental Guidance and Technical Tips

• The immunogen (amino acids 339–565) encompasses the YTH domain critical for m6A recognition; for Western blot, consider using knockout cell lines to confirm band specificity, as this antibody has been KO validated.
• For immunoprecipitation (IP), the antibody may be suitable for enriching endogenous YTHDF2–RNA complexes; include appropriate negative controls (e.g., IgG) to assess background.
• In immunofluorescence/ICC, YTHDF2 typically appears in cytoplasmic foci (P-bodies and stress granules) and can shuttle to the nucleus; optimize fixation/permeabilization conditions for your cell type and treatment (e.g., arsenite-induced stress granules).
• For ELISA, standard indirect ELISA protocols with recombinant YTHDF2 or cell lysates may be employed; validate in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with a streamlined, one-stop channel for sourcing high-quality IVD raw materials. For targets like YTHDF2 involved in epitranscriptomics and RNA modification pathways, we offer validated antibodies, ancillary reagents, and custom procurement services to accelerate your assay development from concept to clinic.