Anti-YTHDF1 Polyclonal Antibody for WB, IHC-P, ELISA - Q9BYJ9

Core Product Specifications and Parameters

Biological Background: YTHDF1 Function and Localization

• YTHDF1 (YTH domain-containing family protein 1), also known as Dermatomyositis associated with cancer putative autoantigen 1 (DACA-1), is a member of the YTHDF paralog family (YTHDF1, YTHDF2, YTHDF3) that specifically binds N6-methyladenosine (m6A)-modified mRNAs.
• Recognizes and binds m6A-containing mRNAs to regulate their stability and translation (PubMed:24284625, PubMed:26318451, PubMed:32492408). Related references: PMID:24284625
  PMID:26318451
  PMID:32492408
• The YTHDF proteins act redundantly to mediate m6A-dependent mRNA degradation and cellular differentiation via the CCR4-NOT complex (PubMed:28106072, PubMed:32492408). Related references: PMID:28106072
  PMID:32492408
• Localizes to the cytoplasm, where it can partition into membraneless compartments such as P-bodies and stress granules through liquid-liquid phase separation upon binding polymethylated mRNAs (PubMed:31292544, PubMed:31388144, PubMed:32451507). Related references: PMID:31292544
  PMID:31388144
  PMID:32451507
• Plays a role in learning and memory formation in the hippocampus by binding m6A-containing neuronal mRNAs (By similarity).
• Acts as a regulator of axon guidance via ROBO3 transcript binding (By similarity) and negatively regulates antigen cross-presentation in dendritic cells, which may limit anti-tumor immune responses (By similarity).
• The gene YTHDF1 undergoes alternative splicing, producing isoforms with distinct functional properties.
• Post-translationally modified by acetylation, phosphorylation, and ubiquitination (keywords: Acetylation, Phosphoprotein, Ubl conjugation).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 280-390 within the C-terminal region of human YTHDF1, which includes part of the YTH domain; consider using it for Western blot (WB) detection of endogenous YTHDF1 (predicted ~61 kDa) across human, mouse, and rat samples.
• For immunohistochemistry (IHC-P), validate antibody specificity in formalin-fixed paraffin-embedded tissues with appropriate controls, as YTHDF1 cytoplasmic localization may require antigen retrieval optimization.
• In ELISA applications, the polyclonal nature may offer broad epitope recognition, but ensure cross-reactivity evaluation with other YTHDF family members if needed.

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