Anti-Ythdc1 Monoclonal Antibody (KO Validated) for WB - E9Q5K9

Core Product Specifications and Parameters

Biological Background: Ythdc1 Function and Localization

• Ythdc1 (YTH domain-containing protein 1) is a nuclear reader of N6-methyladenosine (m6A), a prevalent internal mRNA modification, and specifically recognizes and binds m6A-containing RNAs. Related references: PMID:29262316, PMID:29799838
• It acts as a key regulator of alternative splicing; it promotes exon inclusion by recruiting splicing factor SRSF3 to mRNA sites adjacent to m6A, while inhibiting exon skipping via competition with SRSF10.
• Ythdc1 facilitates nuclear export of m6A-modified mRNAs through interaction with SRSF3 and the export factor NXF1.
• It contributes to S-adenosyl-L-methionine homeostasis by regulating the expression of MAT2A, likely through binding m6A-methylated MAT2A transcripts. Related references: PMID:29262316
• Ythdc1 is essential for germline development: it is required for spermatogonial development in males and oocyte growth and maturation in females, at least in part through its role in alternative splicing. Related references: PMID:29799838
• Independently of its m6A reader activity, Ythdc1 protects the transcription factor PPARG from ubiquitin-mediated proteasomal degradation by preventing its interaction with the E3 ligase ARIH2, thereby supporting brown adipose tissue development and energy homeostasis. Related references: PMID:40355558
• Ythdc1 is localized predominantly in the nucleus and concentrates in nuclear speckles, consistent with its functions in mRNA processing and splicing.
• Additional reported functions include binding m6A on Xist RNA to promote X chromosome inactivation and recognizing m6A on single-stranded DNA; the protein is also subject to phosphorylation and ubiquitin-like modifications.

Experimental Guidance and Technical Tips

• This antibody targets the C-terminal region (aa 597–736) of mouse Ythdc1, which is highly conserved in human YTHDC1; cross-reactivity with human samples is expected.
• For Western blotting, use standard SDS-PAGE and PVDF membrane transfer under reducing conditions. A band near 85 kDa should be observed; post-translational modifications may cause slight shifts.
• As a KO-validated antibody, it is recommended to include appropriate knockout cell or tissue lysates as negative controls to confirm specificity.
• For ELISA applications, consider using the recombinant protein immunogen for assay setup and optimization.
• Given the sequence conservation, detection in human systems may require adjustment of antibody dilution and incubation conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated provider of high-quality IVD raw materials, supporting diagnostic developers, laboratories, and research institutes from concept to clinic. This anti-Ythdc1 monoclonal antibody, targeting a critical m6A RNA reader involved in splicing and gene regulation, reflects our commitment to supplying validated reagents for epigenetics and RNA biology research. Whether you need optimized antibody pairs, bulk ancillary reagents, or assistance with rare target sourcing, CamelBio is your reliable partner for all raw-material needs.