Anti-YPEL5 Polyclonal Antibody for WB, ELISA - P62699

Core Product Specifications and Parameters

Biological Background: YPEL5 Function and Localization

• YPEL5 is a member of the yippee-like protein family, encoded by the YPEL5 gene, and is annotated as a zinc-binding phosphoprotein.
• It functions as a component of the CTLH E3 ubiquitin-protein ligase complex, which selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1. Related references: PMID:29911972
• YPEL5 is required for normal cell proliferation.
• The protein localizes to multiple subcellular structures including the nucleus, cytoplasm, centrosome, spindle pole, and midbody, suggesting involvement in cell division and cytoskeletal organization.
• YPEL5 contains a zinc-coordinating motif, indicative of structural roles or potential DNA-binding capability.
• It is classified as a reference proteome member, supported by mass spectrometry and antibody-based evidence.

Experimental Guidance and Technical Tips

• This antibody was raised against a recombinant protein encompassing the N-terminal 1–121 amino acids of human YPEL5. It is expected to recognize full-length endogenous YPEL5 (~14 kDa) in denaturing Western blot analysis. Consider including positive and negative cell lysates to confirm band specificity.
• For ELISA applications, the antibody may be used as a capture or detection reagent; optimize coating concentrations and blocking conditions empirically.
• Cross-reactivity is reported for human, mouse, and rat; however, due to sequence similarities, reactivity with other species may be possible and should be validated in the relevant experimental system.
• When detecting YPEL5, note that its localization is cell-cycle dependent; synchronization protocols or fractionation assays may be required to observe specific staining patterns.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated one-stop sourcing partner for IVD raw materials, supporting diagnostic developers and research laboratories with validated antibodies, bulk reagents, and custom procurement. This anti-YPEL5 polyclonal antibody is part of our extensive catalog targeting ubiquitination and cell cycle regulation pathways. Whether you need optimized polyclonal or monoclonal antibodies, antibody pairs, or ancillary reagents, our team can help streamline your supply chain from concept to clinic.