Anti-YPEL3 Polyclonal Antibody for WB, ELISA - P61236

Core Product Specifications and Parameters

Biological Background: YPEL3 Function and Localization

• YPEL3 (Protein yippee-like 3) is a member of the yippee-like protein family, encoded by the YPEL3 gene.
• It plays a role in regulating proliferation and apoptosis, particularly in myeloid precursor cells.
• YPEL3 localizes predominantly to the nucleus, with enrichment in the nucleolus.
• The protein is widely expressed across various tissues, suggesting a broad functional role.
• It is classified as a zinc-binding protein and contains metal-binding domains, indicating potential dependence on zinc ions for structure or function.
• Post-translational modifications include ubiquitin-like (Ubl) conjugation, which may affect stability or localization.
• Alternative splicing generates multiple transcript variants, potentially contributing to functional diversity.
• This antibody, raised against amino acids 1-119 (the full-length protein), may recognize all reported isoforms.

Experimental Guidance and Technical Tips

• The immunogen encompasses the full-length YPEL3 sequence (1-119 aa), so the antibody may detect all splice variants if conserved N-terminal epitopes are present; validate band patterns in your system.
• For Western blot, the predicted molecular weight is approximately 14 kDa; use a high-percentage gel (e.g., 12–15% SDS-PAGE) and ensure efficient transfer of low-molecular-weight proteins (e.g., 0.2 µm PVDF membrane).
• Include known positive controls (e.g., cell lines expressing YPEL3) and consider cross-reactivity with mouse/rat homologs when choosing species-specific controls.
• For ELISA applications, optimize coating antigen concentration and antibody dilution; due to polyclonal nature, titration may be necessary to achieve desired sensitivity.
• The observed cross-reactivity with mouse and rat suggests conserved epitopes; take advantage of this for translational models but verify performance empirically.
• Always use fresh reducing sample buffer and boiling for sample preparation to ensure linear epitope exposure for this polyclonal antibody.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your reliable one-stop partner for IVD raw materials, offering a wide range of validated antibodies, optimized recombinant proteins, and bulk ancillary reagents. This anti-YPEL3 polyclonal antibody supports research in apoptosis and cell proliferation, and our dedicated team can assist with custom sourcing of rare-target raw materials, technical consultation, and logistic support to streamline your diagnostic development from concept to clinic.