Anti-YOD1 Rabbit Monoclonal Antibody (WB, ELISA) - Q5VVQ6

Core Product Specifications and Parameters

Biological Background: YOD1 Function and Localization

• YOD1 is a ubiquitin thioesterase belonging to the OTU family of deubiquitinases, with alternative names DUBA-8, OTUD2, and HIN-7.
• It functions as a hydrolase that removes conjugated ubiquitin from proteins, playing a critical role in ER-associated degradation (ERAD) of misfolded lumenal proteins.
• YOD1 may trim ubiquitin chains on substrates to facilitate their passage through the VCP/p97 pore, as ubiquitin moieties can sterically hinder threading.
• The enzyme exhibits linkage specificity, cleaving Lys-27, Lys-29, Lys-33, and Lys-11 linked polyubiquitin chains.
• YOD1 is implicated in macroautophagy, particularly in clearing damaged lysosomes by recruiting PLAA, UBXN6, and VCP to K48-ubiquitinated lysosomal membranes. Related references: PMID:27753622
• It is primarily localized in the cytoplasm and contains a zinc-finger domain, classifying it as a metal-binding thiol protease.
• Alternative splicing of the YOD1 gene produces multiple isoforms, and its molecular weight is approximately 38 kDa.

Experimental Guidance and Technical Tips

• For Western blotting, consider testing a standard antibody dilution range (e.g., 1:500-1:2000) and optimize using lysates from your specific cell or tissue system expressing YOD1.
• Since the immunogen is a peptide from the OTU domain (aa 249-348), confirm the antibody’s specificity by including YOD1 knockout or siRNA knockdown controls.
• ELISA performance may require optimization of coating antigen concentration and antibody incubation conditions; preliminary checkerboard titrations are recommended.
• The predicted molecular weight of YOD1 is ~38 kDa; a band near this size on SDS-PAGE under reducing conditions is expected, but post-translational modifications or isoforms could shift the apparent mass.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic and research laboratories with a comprehensive sourcing solution, from validated antibody pairs and optimized monoclonal antibodies to bulk ancillary reagents. For targets like YOD1 involved in ubiquitin-mediated proteolysis and ER stress, we offer customized raw-material procurement to accelerate your assay development and production pipelines. Our one-stop model supports every stage from concept to clinic, making us your reliable partner for IVD raw material supply.