Anti-YME1L1 Rabbit Polyclonal Antibody for WB, ELISA - Q96TA2

Core Product Specifications and Parameters

Biological Background: YME1L1 Function and Localization

• YME1L1 is an ATP-dependent zinc metalloprotease (also known as FtsH1, Meg-4, PAMP) localized to the mitochondrial inner membrane, with high expression in cardiac and skeletal muscle.
• It catalyzes the degradation of folded and unfolded proteins bearing degron sequences within the mitochondrial intermembrane space, playing a central role in mitochondrial protein quality control. Related references: PMID:24315374, PMID:26923599, PMID:27786171
• YME1L1 regulates mitochondrial morphology by cleaving the dynamin-like GTPase OPA1 at site S2, generating a short isoform necessary for mitochondrial fusion and cristae maintenance. Related references: PMID:18076378, PMID:26923599, PMID:27495975
• It supports cell proliferation, complex I respiration, and antiapoptotic activity by removing oxidatively damaged membrane proteins and unassembled respiratory chain subunits such as NDUFB6, OX4, and ND1. Related references: PMID:22262461
• In response to nutrient starvation, YME1L1 limits mitochondrial biogenesis through mTORC1-LPIN1-dependent activation, leading to degradation of translocases, lipid transfer proteins, and metabolic enzymes. Related references: PMID:31695197
• YME1L1 mediates the degradation of specific substrates including OMA1 (upon membrane depolarization), TIMM17A (downstream of the integrated stress response), MICU1 (when non-oxidized), PRELID1, and the STARD7 targeting sequence, thereby modulating mitochondrial stress responses, protein import, calcium signaling, and lipid metabolism. Related references: PMID:26923599, PMID:24315374, PMID:36206740, PMID:27495975, PMID:29301859
• YME1L1 dysfunction is associated with optic atrophy type 11 (OPA11), and the protease is critical for adult neural stem cell self-renewal (By similarity).

Experimental Guidance and Technical Tips

• Since the antibody is raised against the N-terminal region (aa 1-240) of human YME1L1 and shows cross-reactivity with mouse and rat, consider sequence alignment of the target epitope across species to anticipate reactivity.
• For Western blotting, YME1L1 runs at approximately 86 kDa; mitochondrial protein extracts are recommended for detection given its submitochondrial localization.
• ELISA applications may benefit from using recombinant YME1L1 protein or peptide as a coating antigen to optimize binding conditions.
• As a polyclonal antibody, lot-to-lot variability may occur; validate new lots in your experimental setup.
• Consider performing a dose-response curve with purified protein to determine optimal antibody dilution for your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. This YME1L1 antibody supports research into mitochondrial quality control mechanisms and related disease areas, and is offered alongside our comprehensive portfolio of mitochondrial research tools. From rare target sourcing to scalable manufacturing, CamelBio serves as your reliable partner from concept to clinic.