Anti-YME1L1 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q96TA2

Core Product Specifications and Parameters

Biological Background: YME1L1 Function and Localization

• ATP-dependent zinc metalloprotease YME1L1 (alias FTSH1, MEG4, PAMP) is a member of the AAA ATPase family and functions primarily in the mitochondrial inner membrane.
• YME1L1 catalyzes the degradation of both folded and unfolded proteins with degron sequences in the mitochondrial intermembrane region, playing a key role in mitochondrial protein quality control.
  Related references: PMID:24315374, PMID:26923599, PMID:27786171
• It cleaves OPA1 at site S2, generating a short form essential for maintaining normal mitochondrial cristae morphology and protein metabolism.
  Related references: PMID:18076378, PMID:26923599, PMID:27495975
• YME1L1 ensures cell proliferation, maintains complex I respiration activity, promotes antiapoptotic activity, and protects mitochondria from accumulating oxidatively damaged membrane proteins.
  Related references: PMID:22262461
• In response to nutrient starvation or stress, YME1L1 mediates mitochondrial proteome rewiring by degrading translocases, lipid transfer proteins, and metabolic enzymes, regulated by mTORC1 and LPIN1-mediated phosphatidylethanolamine levels.
  Related references: PMID:31695197
• Subcellular localization is the mitochondrion inner membrane, with high expression in cardiac and skeletal muscle mitochondria.
• Additional substrates of YME1L1 include OMA1 (degraded upon membrane depolarization), PRELID1, TIMM17A, MICU1, and STARD7, highlighting its broad role in mitochondrial homeostasis.
  Related references: PMID:26923599, PMID:27495975, PMID:24315374, PMID:36206740, PMID:29301859

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 580-773) lies within a conserved region of the AAA ATPase domain; this may contribute to specific target recognition.
• For western blotting, the predicted molecular weight is approximately 86kDa; validate in relevant mitochondrial fractions or whole-cell lysates.
• For immunofluorescence/immunocytochemistry, mitochondrial inner membrane localization suggests a punctate staining pattern; consider using mitochondrial counterstains (e.g., MitoTracker) for co-localization.
• For ELISA applications, ensure optimal antigen presentation; the antibody is applicable to human samples; cross-reactivity with other species should be validated if required.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials and technical services, covering every stage from concept to clinic. This YME1L1 Rabbit mAb supports research into mitochondrial biology, dynamics, and related diseases. CamelBio can assist with sourcing validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw materials.