Anti-YKL-40/CHI3L1 Rabbit Monoclonal Antibody for WB, IP, IHC-P, ELISA - Q61362

Core Product Specifications and Parameters

Biological Background: YKL-40/CHI3L1 Function and Localization

• Encodes multiple isoforms via alternative initiation and alternative splicing.
• Lacks chitinase activity but functions as a carbohydrate-binding lectin with preference for chitin; involved in tissue remodeling and cellular adaptation to environmental changes.
• Plays a key role in Th2 inflammatory responses and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation, and M2 macrophage differentiation.
• Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs; mediates AKT1 signaling and IL8 production in colonic epithelial cells.
• In lung, regulates antibacterial responses by enhancing macrophage bacterial killing, controlling bacterial dissemination, and augmenting host tolerance; also regulates hyperoxia-induced lung injury, inflammation, and epithelial apoptosis.
• Subcellular localization includes secretion into extracellular space, as well as presence in cytoplasm and endoplasmic reticulum.
• Tissue specificity: detected in lung (pulmonary macrophages, alveolar type 2 cells, bronchoalveolar lavage fluid), and in mammary tumor cells; not detected in non-inflammatory colon.
• Annotated keywords: 3D-structure, Alternative initiation, Alternative splicing, Antimicrobial, Apoptosis, Cytoplasm, Disulfide bond, Endoplasmic reticulum, Glycoprotein, Inflammatory response, Reference proteome, Secreted, Signal.

Experimental Guidance and Technical Tips

• Based on the immunogen design spanning amino acids 30-389 of mouse Chi3l1, this antibody may recognize multiple isoforms and is potentially suitable for detecting both full-length and alternative splice variants; consider sequence alignment for cross-reactivity with other species.
• For WB: predicted molecular weight around 43-44 kDa under reducing conditions, but may migrate differently due to glycosylation or alternative initiation; include positive controls such as lung tissue lysates or macrophage cell lines.
• For IHC-P: antigen retrieval may be required; validate in tissues with known expression (e.g., inflamed lung, mammary tumor) and include negative control (non-inflammatory colon).
• For IP and ELISA: ensure buffer compatibility; consider using antibody at recommended concentrations and validate in the relevant sample system.
• Cross-reactivity is reported for mouse; for other species, verify sequence homology and perform appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

This YKL-40/CHI3L1 antibody supports research in inflammation, tissue remodeling, and host-pathogen interactions. As a specialized IVD raw-material partner, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and efficient sourcing for rare targets to help streamline your diagnostic development from concept to clinic.