Anti-YIPF7 Polyclonal Antibody for WB and ELISA - Q8N8F6

Core Product Specifications and Parameters

Biological Background: YIPF7 Function and Localization

• Protein YIPF7 (also known as FinGER9, YIP1 family member 7) is a member of the YIP1 family, implicated in vesicular trafficking between the endoplasmic reticulum and Golgi apparatus.
• It is a five-pass transmembrane protein localized to both the endoplasmic reticulum and Golgi apparatus membranes, including cis- and trans-Golgi network.
• The YIPF7 gene encodes a protein of approximately 31 kDa (calculated molecular weight ~27.8 kDa from UniProt) that undergoes alternative splicing.
• Subcellular localization studies indicate YIPF7 is present in the ER, cis-Golgi, and trans-Golgi, suggesting a role in early secretory pathway organization or cargo transport.
• As a member of the YIP1 family, YIPF7 may interact with Rab GTPases or other trafficking machinery components, though specific functions remain under investigation.
• Keywords: Alternative splicing, Endoplasmic reticulum, Golgi apparatus, Membrane, Transmembrane helix.

Experimental Guidance and Technical Tips

• As the immunogen corresponds to the N-terminal 1-80 amino acids of human YIPF7, the antibody may recognize both full-length and potential splice variants if epitopes are conserved.
• For Western blot, consider using whole-cell lysates from human or rat cell lines, as cross-reactivity to rat is indicated. Validate detection conditions in your specific sample system.
• For ELISA, immobilize the recombinant protein or peptide to capture the antibody; optimize coating buffer and blocking conditions for best signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target raw-material procurement. Whether you are advancing research in Golgi/ER trafficking or developing diagnostic assays for YIPF7-related targets, we offer a streamlined supply chain to support your project from concept to clinic.