Anti-YEATS4 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O95619

Core Product Specifications and Parameters

Biological Background: YEATS4 Function and Localization

• YEATS4 is a chromatin reader component of the NuA4 histone acetyltransferase (HAT) complex, which acetylates nucleosomal histones H4 and H2A to activate transcription. Related references: PMID:12963728 PMID:14966270
• It specifically recognizes and binds diacetylated histone H3 marks, particularly H3K18ac/H3K27ac and H3K14ac/H3K27ac, and plays a key role in histone variant H2AZ1 deposition at promoters of actively transcribed genes. Related references: PMID:29437725 PMID:29900004 PMID:30071723
• YEATS4 can also bind crotonylated histone H3 and may recognize histone H3 succinylated at Lys-122 (H3K122succ). Related references: PMID:30071723 PMID:29463709
• It localizes to the nucleus, consistent with its chromatin regulatory functions.
• YEATS4 is broadly expressed in tissues including brain, heart, kidney, liver, lung, pancreas, placenta, and skeletal muscle.
• The protein contains a coiled-coil domain and is subject to post-translational modifications including ubiquitination (isopeptide bond formation).

Experimental Guidance and Technical Tips

• The immunogen spans the full-length YEATS4 protein (aa 1–227), suggesting the antibody may recognize native and denatured forms; confirm specificity by knockdown or knockout controls where possible.
• For WB, an expected band near 26 kDa should be observed; optimize loading amounts and transfer conditions for clear signals.
• For IHC-P and IF/ICC, consider antigen retrieval methods and validate staining patterns against known nuclear localization.
• In ELISA, ensure the coating antigen presents the YEATS4 epitopes recognized by this polyclonal antibody; non-specific binding should be controlled with appropriate buffers.
• Cross-reactivity with mouse and rat YEATS4 has been reported; verify band sizes and expression patterns in tissues from these species.

CamelBio: Your One-Stop Sourcing Bridge

As a specialized IVD raw-material provider, CamelBio offers comprehensive access to validated antibodies, recombinant proteins, and ancillary reagents for chromatin and transcription research. This anti-YEATS4 polyclonal antibody exemplifies our capability in sourcing high-quality detection tools for epigenetic targets. Explore our full portfolio for diagnostic development, including bulk formats, custom technical support, and rare-target procurement services.