Anti-YBEY Rabbit Polyclonal Antibody for WB and ELISA - P58557

Core Product Specifications and Parameters

Biological Background: YBEY Function and Localization

• YBEY, also known as C21orf57, is an endoribonuclease encoded by the YBEY gene in humans.
• It functions as a single-strand-specific metallo-endoribonuclease involved in rRNA maturation.
• The protein contains a conserved zinc-binding motif and belongs to the RNase YbeY family.
• Subcellular localization is primarily nuclear, consistent with its role in ribosome biogenesis.
• Alternative splicing produces multiple isoforms, contributing to functional diversity.
• Keywords: Endonuclease, Hydrolase, Metal-binding, Nuclease, Nucleus, Zinc, Reference proteome, Alternative splicing.
• The enzyme is evolutionarily conserved and critical for maintaining translational accuracy.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide spanning amino acids 68–167; pre-incubating with the immunizing peptide can confirm band specificity in WB.
• For WB, denaturing and reducing conditions typically yield a band near the predicted 19 kDa; validate with appropriate positive controls in your sample system.
• In ELISA, consider the antigen immobilization format and antibody concentration to optimize signal-to-noise ratio.
• Cross-reactivity with rat indicates epitope conservation; validation in rat-derived samples is recommended for intended applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized recombinant antibodies, and bulk ancillary reagents, to support your diagnostic development from concept to clinic. Our YBEY polyclonal antibody complements our range of nucleic acid-binding protein reagents and can be integrated with our custom sourcing services for rare targets. Partner with us to streamline your supply chain and accelerate the development of robust diagnostic assays.