Anti-YB-1/YBX1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P67809

Core Product Specifications and Parameters

Biological Background: YB-1/YBX1 Function and Localization

• YB-1/YBX1 is a multifunctional DNA- and RNA-binding protein implicated in translational repression, mRNA stabilization, splicing, DNA repair, and transcriptional regulation. Related references: PMID:10817758, PMID:11698476, PMID:14718551
• As an RNA-binding protein, YB-1 preferentially recognizes 5'-[CU]CUGCG-3' motifs and m5C-modified transcripts, and promotes mRNA stability by recruiting ELAVL1. Related references: PMID:10817758, PMID:11698476, PMID:19561594, PMID:31358969
• YB-1 plays a key role in exosome RNA sorting, directing small non-coding RNAs (tRNAs, Y RNAs, miRNAs) into exosomes by recognizing m5C-modified RNAs. Related references: PMID:27559612, PMID:29073095, PMID:28341602
• It regulates alternative pre-mRNA splicing by binding to splice sites and modulating splice site selection. Related references: PMID:12604611
• YB-1 also binds DNA at Y-box sequences (e.g., in MDR1 and HLA class II promoters) to regulate transcription, and has endonucleolytic activity possibly involved in DNA repair. Related references: PMID:8188694, PMID:18809583, PMID:14718551
• Subcellularly, YB-1 localizes to the cytoplasm, nucleus, cytoplasmic granules, P-bodies, and is secreted via exosomes; it can act as an extracellular mitogen. Post-translational modifications include acetylation, phosphorylation, and ubiquitination. Related references: PMID:19483673

Experimental Guidance and Technical Tips

• The immunogen (amino acids 1-55) covers the N-terminal region of human YB-1, which is highly conserved across human, mouse, and rat, supporting the observed cross reactivity.
• For Western blotting, a band around 36 kDa is expected; verify expression in relevant cell lines or tissues.
• In IF/ICC, permeabilization is necessary for nuclear detection; consider co-staining with organelle markers.
• For ELISA, the antibody may be used with recombinant YB-1 or cell lysates; optimize coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. For YB-1-related studies, we offer validated antibody pairs, bulk ancillary reagents, and customized polyclonal antibody development to accelerate your research and diagnostic kit development. With expertise in rare target sourcing and one-stop supply chain management, CamelBio bridges your raw material needs seamlessly.