Anti-YB-1/YBX1 Rabbit Polyclonal Antibody for WB, IF, IHC, ELISA - P67809

Core Product Specifications and Parameters

Biological Background: YB-1/YBX1 Function and Localization

• YB-1/YBX1 is a multifunctional DNA- and RNA-binding protein involved in translational repression, RNA stabilization, mRNA splicing, DNA repair, and transcription regulation. Related references: PMID:10817758, PMID:11698476, PMID:14718551
• It binds preferentially to the 5'-[CU]CUGCG-3' RNA motif and specifically recognizes m5C-modified mRNAs, promoting mRNA stabilization by recruiting ELAVL1. Related references: PMID:19561594, PMID:31358969
• Acts as a component of the CRD-mediated complex that enhances MYC mRNA stability. Related references: PMID:19029303
• Plays a key role in sorting small non-coding RNAs (tRNAs, Y RNAs, miRNAs) into extracellular exosomes by recognizing m5C-containing RNAs. Related references: PMID:27559612, PMID:29073095, PMID:28341602
• Regulates alternative splicing by binding to splice sites in pre-mRNA and modulating splice site selection. Related references: PMID:12604611
• Binds to Y-box promoter elements (5'-CTGATTGGCCAA-3') to regulate transcription of MDR1 and HLA class II genes, and promotes separation of cisplatin-modified DNA strands, contributing to DNA repair. Related references: PMID:8188694, PMID:18809583, PMID:14718551
• The secreted form functions as an extracellular mitogen, stimulating cell migration and proliferation. Related references: PMID:19483673
• Subcellular localization spans cytoplasm, nucleus, cytoplasmic granules, P-bodies, and secretion via exosomes.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen (aa 100-200) targets a conserved central region of human YB-1/YBX1; cross-reactivity with mouse and rat suggests sequence conservation, but verify reactivity in your specific model system.
• For Western blot, expect a band at ~36 kDa, though post-translational modifications (acetylation, phosphorylation) may cause shifts. Optimize blocking and antibody titration.
• In immunofluorescence and immunohistochemistry, both nuclear and cytoplasmic signals should be expected; include appropriate permeabilization and antigen retrieval steps.
• For ELISA, consider pairing with a detection antibody that recognizes a non-overlapping epitope to ensure specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated IVD raw-material supplier, offering a comprehensive portfolio of antibodies, antigens, and reagents to support your assay development from concept to clinic. This YB-1/YBX1 polyclonal antibody is suitable for studying RNA biology, exosome research, and cancer diagnostics. CamelBio can also assist in sourcing matched antibody pairs, recombinant proteins, and custom assay components for your specific workflow.