Anti-YB-1/YBX1 Rabbit Monoclonal Antibody for WB, IP, IF/ICC, IHC-P, ELISA - P67809

Core Product Specifications and Parameters

Biological Background: YB-1/YBX1 Function and Localization

• YB-1 is a DNA- and RNA-binding protein involved in translational repression, RNA stabilization, mRNA splicing, DNA repair, and transcription regulation (PubMed:10817758, PubMed:11698476, PubMed:14718551). Related references: PMID:10817758 PMID:11698476 PMID:14718551
• It preferentially binds to 5'-[CU]CUGCG-3' RNA motifs and m5C-modified mRNAs, recruiting ELAVL1 to promote mRNA stabilization (PubMed:19561594, PubMed:31358969). Related references: PMID:19561594 PMID:31358969
• As a component of the CRD-mediated complex, YB-1 enhances MYC mRNA stability (PubMed:19029303). Related references: PMID:19029303
• It sorts small non-coding RNAs (tRNAs, Y RNAs, miRNAs) into exosomes by recognizing m5C-containing RNAs (PubMed:27559612, PubMed:29073095, PubMed:28341602). Related references: PMID:27559612 PMID:29073095 PMID:28341602
• YB-1 prevents epidermal progenitor senescence by regulating translation of senescence-associated cytokine mRNAs (PubMed:29712925). Related references: PMID:29712925
• It binds to Y-box motifs in promoters (e.g., MDR1, HLA class II) to regulate transcription, enhanced by acetylated APEX1 (PubMed:18809583, PubMed:8188694). Related references: PMID:18809583 PMID:8188694
• The secreted form acts as an extracellular mitogen, stimulating cell migration and proliferation (PubMed:19483673). Related references: PMID:19483673
• YB-1 localizes to the cytoplasm, nucleus, cytoplasmic granules, P-bodies, and secreted exosomes.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to amino acids 225-324, an internal region of human YB-1. This region may be suitable for detecting total YB-1 protein.
• This antibody has been tested in WB, IP, IF/ICC, IHC-P, and ELISA. Start with recommended dilutions and optimize for your experimental conditions.
• Cross-reactivity is observed with human, mouse, and rat YB-1. Verify specificity in your sample system using appropriate controls.
• Consider using cell lines with known YB-1 expression as positive controls and knockout or knockdown models as negative controls.
• YB-1 is subject to post-translational modifications; epitope accessibility may vary. Validate detection of modified forms if relevant.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your trusted one-stop partner for IVD raw material sourcing. Our anti-YB-1/YBX1 rabbit monoclonal antibody supports studies on RNA biology, transcriptional regulation, and DNA repair. We provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and can assist in sourcing rare targets to accelerate your diagnostic development from concept to clinic.