Anti-YAP1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P46937

Core Product Specifications and Parameters

Biological Background: YAP1 Function and Localization

• YAP1 (Yes-associated protein 1) is a transcriptional coactivator and corepressor, also known as YAP65 and protein yorkie homolog, playing a dual regulatory role in gene expression.
• YAP1 is a critical downstream target of the Hippo signaling pathway, which is essential for organ size control and tumor suppression by restricting cell proliferation and promoting apoptosis. Related references: PMID:17974916 PMID:18280240 PMID:18579750
• Phosphorylation of YAP1 by LATS1/2 kinases inhibits its nuclear translocation, thereby controlling the expression of genes involved in cell growth and EMT. Related references: PMID:18158288 PMID:26598551 PMID:34404733
• YAP1 transcriptional activity requires interaction with TEAD transcription factors, modulating anchorage-independent growth and epithelial-mesenchymal transition (EMT). Related references: PMID:18579750
• YAP1 contributes to tissue tension and 3D tissue shape by regulating the cortical actomyosin network through ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization. Related references: PMID:25778702
• YAP1 suppresses ciliogenesis by acting as a transcriptional corepressor of TEAD4 target genes AURKA and PLK1. Related references: PMID:25849865
• YAP1 localizes to the cytoplasm, nucleus, cell junctions, tight junctions, and cell membrane, and its expression is elevated in certain liver and prostate cancers.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 155-504 of human YAP1, encompassing the TEAD-binding domain and part of the transactivation domain; consider validating detection of YAP1 isoforms and phosphorylation states.
• For Western blotting, YAP1 is detected at approximately 54 kDa; pre-validate the antibody in your specific cell or tissue lysate to ensure specificity for endogenous or overexpressed protein.
• In immunohistochemistry (IHC-P), antigen retrieval methods may influence staining; perform pilot experiments to determine optimal conditions.
• For immunofluorescence (IF/ICC), confirm subcellular localization patterns (cytoplasmic/nuclear) under different growth conditions, as YAP1 shuttles upon Hippo pathway activation.
• The antibody is applicable to immunoprecipitation (IP); titrate the antibody amount and use appropriate negative controls to minimize non-specific binding.
• When using ELISA, test various coating conditions and antibody dilutions to achieve optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop platform for diagnostic manufacturers and research laboratories, offering streamlined access to high-quality IVD raw materials. Our catalog includes expertly validated YAP1 antibodies like this polyclonal antibody, alongside customized monoclonal antibody pairs and bulk ancillary reagents, all designed to support your Hippo pathway and oncology research workflows. With robust supply capabilities and technical consultation, CamelBio simplifies procurement from discovery to clinical application, ensuring you obtain the precise raw materials you need for reliable immunoassay development.