Anti-YAP1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P46937

Core Product Specifications and Parameters

Biological Background: YAP1 Function and Localization

• Transcriptional coactivator and corepressor that serves as a critical downstream effector of the Hippo signaling pathway, controlling organ size, proliferation, and apoptosis. Related references: PMID:17974916 PMID:18280240 PMID:18579750
• In the Hippo pathway, MST1/2 kinases phosphorylate LATS1/2, which then phosphorylate YAP1 at multiple sites, preventing its nuclear translocation and promoting cytoplasmic retention. Related references: PMID:18158288 PMID:26598551 PMID:34404733
• Nuclear YAP1 interacts with TEAD transcription factors to drive expression of genes involved in cell growth, anchorage-independent growth, and epithelial-mesenchymal transition (EMT). Related references: PMID:18579750
• Regulates tissue tension and 3D tissue shape by modulating the cortical actomyosin network through ARHGAP18, a Rho GTPase-activating protein that suppresses F-actin polymerization. Related references: PMID:25778702
• Acts as a transcriptional corepressor of TEAD4 to suppress ciliogenesis by repressing expression of AURKA and PLK1. Related references: PMID:25849865
• Localizes to the cytoplasm, nucleus, tight junctions, and cell membrane; shuttles between compartments in response to phosphorylation and cell density.
• Overexpressed in some liver and prostate cancers; isoforms lacking the transactivation domain are found in striatal neurons of Huntington disease patients.
• Post-translationally modified by phosphorylation, ubiquitination, and alternative splicing; functions as both activator and repressor, and is classified as a proto-oncogene.

Experimental Guidance and Technical Tips

• The immunogen (residues 155–504) covers multiple regulatory domains including TEAD-binding motifs and portions of the transactivation domain; the antibody may recognize full-length YAP1 and some splice isoforms.
• For Western blot, a predominant band near 54 kDa is expected; however, additional bands may appear due to phosphorylation, ubiquitination, or isoform variation.
• Validate antibody specificity in your model system using siRNA knockdown or Hippo pathway modulators (e.g., serum starvation to induce YAP1 phosphorylation and cytoplasmic retention).
• For immunofluorescence, note that YAP1 localization is cell-density dependent; under sparse conditions it is nuclear, while under high confluence it becomes cytoplasmic.
• Optimize antigen retrieval for IHC-P and include appropriate controls (e.g., tissue known to overexpress YAP1, such as certain carcinomas).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic developers and researchers, offering validated antibodies, optimized polyclonal and monoclonal reagents, bulk ancillary materials, and rare-target sourcing. This anti-YAP1 rabbit polyclonal antibody supports studies in Hippo signaling and oncology, and complements a full portfolio of cell signaling antibodies and related raw materials. Trust CamelBio to provide flexible, high-quality sourcing solutions that accelerate your diagnostic development from concept to clinic.