Anti-YAP1 Rabbit Monoclonal Antibody for WB - P46937

Core Product Specifications and Parameters

Biological Background: YAP1 Function and Localization

• Transcriptional coactivator and corepressor that acts as a critical downstream effector of the Hippo signaling pathway, regulating organ size and tumor suppression by restricting proliferation and promoting apoptosis. Related references: PMID:17974916, PMID:18280240, PMID:18579750
• Key component of the Hippo kinase cascade: STK3/MST2 and STK4/MST1, with SAV1, phosphorylate and activate LATS1/2-MOB1 complexes, which then phosphorylate YAP1, leading to its inactivation and cytoplasmic retention. Related references: PMID:18158288
• Phosphorylation by LATS1/2 at multiple sites prevents nuclear translocation, thereby inhibiting YAP1-driven transcription of target genes via TEAD transcription factors, which modulates cell growth, anchorage-independent growth, and epithelial-mesenchymal transition (EMT). Related references: PMID:18158288, PMID:26598551, PMID:34404733, PMID:18579750
• Regulates tissue tension and 3D tissue morphology by suppressing F-actin polymerization through the Rho GTPase activating protein ARHGAP18. Related references: PMID:25778702
• Functions as a transcriptional corepressor of TEAD4 to suppress ciliogenesis by repressing AURKA and PLK1 expression. Related references: PMID:25849865
• Synergizes with WBP2 to enhance progesterone receptor (PGR) activity. Related references: PMID:16772533
• Increased expression is observed in some liver and prostate cancers; isoforms lacking the transactivation domain are found in striatal neurons of Huntington disease patients (at protein level).
• Localizes to the cytoplasm, nucleus, cell junctions (tight junctions), and cell membrane, reflecting its dynamic shuttling and multiple functional contexts.

Experimental Guidance and Technical Tips

• As a rabbit monoclonal antibody raised against a recombinant fusion protein spanning amino acids 155–504 (NP_001123617.1), it covers the central and C-terminal domains of human YAP1, potentially recognizing multiple isoforms. Consider validating isoform detection in your sample type.
• For Western blotting, the predicted molecular weight is approximately 54 kDa; use appropriate loading controls and confirm specificity with knockout (KO) cell lysates where possible.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), antigen retrieval methods and antibody dilution may need optimization; validate in relevant tissue sections with proper controls.
• For chromatin immunoprecipitation (ChIP) experiments, verify enrichment of known TEAD-factor target promoters to ensure functional binding in your system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for IVD raw materials, offering diagnostic developers and research teams streamlined access to validated antibodies, including this YAP1 rabbit monoclonal antibody for Hippo pathway and cancer research applications. We support your projects with optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets—empowering your workflow from concept to clinic.