Anti-Y14/RBM8A Rabbit Monoclonal Antibody for WB, ELISA - Q9Y5S9

Core Product Specifications and Parameters

Biological Background: Y14/RBM8A Function and Localization

• Y14/RBM8A, also called RNA-binding protein 8A or BOV-1, is a core component of the exon junction complex (EJC) and is required for pre-mRNA splicing as part of the spliceosome. Related references: PMID:28502770 PMID:29301961
• It forms a heterodimer with MAGOH, which inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation.
• The MAGOH-RBM8A heterodimer interacts with PYM1 to facilitate EJC disassembly in the cytoplasm, enhancing translation of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex.
• As part of the EJC, RBM8A is involved in nonsense-mediated mRNA decay (NMD) and influences post-transcriptional processes including nuclear export, subcellular localization, and translation efficiency.
• Y14/RBM8A is localized in the nucleus, nuclear speckles, and cytoplasm, reflecting its dynamic roles in mRNA metabolism.
• The protein is ubiquitously expressed across tissues, consistent with its fundamental function in mRNA processing.
• Post-translational modifications include acetylation, phosphorylation, and isopeptide bond formation (Ubl conjugation), with alternative splicing generating multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–100 of human RBM8A, targeting an N-terminal region; verify recognition of known isoforms or splice variants in your sample system.
• For Western blotting, the expected protein weight is ~20 kDa; consider using 12–15% SDS-PAGE gels and appropriate molecular weight markers.
• Validated species reactivity includes human, mouse, and rat; cross-reactivity in other species should be tested if required.
• In ELISA, the antibody may be used as a capture or detection reagent; optimal working concentrations must be determined empirically.
• Include positive controls such as HeLa, HEK293, or other cell lines with confirmed RBM8A expression; loading controls should be chosen accordingly.
• This antibody is suitable for research applications investigating the exon junction complex, spliceosome, or NMD pathways; performance in other assays requires further validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to IVD raw materials like monoclonal antibodies, covering targets involved in RNA biology and post-transcriptional regulation. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support assay development. For rare or custom targets such as Y14/RBM8A, our sourcing services connect you with the materials needed from concept to clinical application.