Anti-XRCC3 Rabbit Polyclonal Antibody for WB and ELISA - O43542

Core Product Specifications and Parameters

Biological Background: XRCC3 Function and Localization

• XRCC3, also known as X-ray repair cross-complementing protein 3, is a DNA repair protein crucial for maintaining genomic stability.
• It is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks, repairing chromosomal fragmentation, translocations, and deletions.
• XRCC3 is a component of the RAD51 paralog protein complex CX3, which functions in the BRCA1-BRCA2-dependent HR pathway downstream of RAD51 recruitment, and binds to Holliday junctions and replication fork junctions.
• The protein plays a role in Holliday junction resolution and processing of HR intermediates, potentially via interaction with GEN1 during mitosis.
• It is part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C, contributing to DNA repair by homologous recombination.
• Under oxidative stress, XRCC3 helps regulate mitochondrial DNA copy number in conjunction with RAD51 and RAD51C.
• Subcellular localization includes the nucleus, cytoplasm, perinuclear region, and mitochondria, reflecting its roles in nuclear DNA repair and mitochondrial DNA maintenance.
• Post-translational modifications include acetylation, and it possesses ATP-binding and DNA-binding domains, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 62-346 of human XRCC3, covering a substantial portion of the protein; this design may provide broad epitope recognition.
• For Western blot, the predicted molecular weight is approximately 38 kDa (consistent with the UniProt-calculated mass of 37.8 kDa). Validate the band size using suitable molecular weight markers and consider using known positive controls (e.g., human cell lines expressing XRCC3).
• In ELISA applications, optimize coating antigen and antibody dilution to ensure specific signal; recombinant XRCC3 protein or peptides can serve as positive controls.
• Cross-reactivity is reported for human; when working with other species, verify sequence homology and perform pilot experiments to confirm reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive channel for IVD raw material procurement, supporting diagnostic manufacturers, laboratories, and research institutions from discovery to clinical application. For DNA repair and genomic stability research, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies like this anti-XRCC3 product, along with bulk ancillary reagents and sourcing for rare targets. Contact us to streamline your supply chain and accelerate project development.