Anti-XRCC2 Polyclonal Antibody for WB, IHC-P, ELISA - O43543

Core Product Specifications and Parameters

Biological Background: XRCC2 Function and Localization

• Encodes DNA repair protein XRCC2, also known as X-ray repair cross-complementing protein 2; aliases include FANCU, POF17, SPGF50.
• Functions in homologous recombination repair (HRR) of double-stranded DNA breaks, crucial for maintaining genomic stability by repairing chromosomal fragmentation, translocations, and deletions.
• Component of the RAD51 paralog protein complex BCDX2 (RAD51B-RAD51C-RAD51D-XRCC2), which operates in the BRCA1-BRCA2-dependent HR pathway.
• Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 loading; it binds predominantly to Holliday junctions and replication fork junctions.
• Subcellular localization includes the nucleus, cytoplasm, and centrosomes (microtubule organizing centers), suggesting roles in DNA repair and possibly cell cycle regulation.
• Post-translational modifications include phosphorylation; implicated in disease variants for Fanconi anemia (complementation group U) and premature ovarian failure 17.
• Molecular weight of the unmodified protein is approximately 32 kDa (calculated 31.96 kDa), consistent with the observed band size in western blotting.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal 1-83 amino acids of human XRCC2; expect detection of a band at ~32 kDa in western blotting.
• Validate the antibody in a suitable positive control sample, such as HeLa or HEK293 cell lysates, as XRCC2 is ubiquitously expressed.
• For IHC-P, consider optimizing antigen retrieval methods (e.g., citrate buffer pH 6.0, heat-induced) and titrating the primary antibody to minimize background.
• Cross-reactivity is reported with mouse and rat; verify reactivity in your experimental species by sequence alignment of the immunogen region.
• This polyclonal antibody can be used for ELISA; ensure proper coating of antigen and use appropriate secondary antibody detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, from concept to clinic. For researchers and manufacturers focusing on DNA repair pathways, we offer a broad range of validated antibodies, antigen pairs, and ancillary reagents. Need a rare specificity or bulk quantities? Contact us to streamline your supply chain for anti-XRCC2 and related DNA damage response targets.