Anti-XRCC1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P18887

Core Product Specifications and Parameters

Biological Background: XRCC1 Function and Localization

• XRCC1 is a scaffold protein that mediates the assembly of DNA break repair protein complexes at sites of single-strand breaks. Related references: PMID:11163244, PMID:28002403
• It negatively regulates the ADP-ribosyltransferase activity of PARP1 during base-excision repair, preventing excessive PARylation. Related references: PMID:28002403, PMID:34102106, PMID:34811483
• XRCC1 specifically recognizes and binds auto-poly-ADP-ribosylated PARP1, limiting its activity. Related references: PMID:14500814, PMID:34102106, PMID:34811483
• Localized predominantly in the nucleus and associated with chromosomes, consistent with its role in DNA repair.
• Expressed in fibroblasts, retinal pigmented epithelial cells, and lymphoblastoid cells at the protein level.
• Post-translationally modified by phosphorylation and ubiquitin-like conjugation (Ubl), contributing to its regulation.
• Associated with neurodegenerative diseases, highlighting its importance in maintaining genomic stability.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 480-633, suggesting the antibody may recognize epitopes in the C-terminal region of XRCC1. Consider using this knowledge for peptide blocking experiments.
• For Western blot (WB), a band around 69 kDa is expected, corresponding to the full-length protein. Validation in the relevant sample system is recommended.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), appropriate fixation and permeabilization methods should be optimized as XRCC1 is a nuclear protein.
• For ELISA, the antibody can be used as a capture or detection reagent; pairing with another XRCC1 antibody may enhance sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials. For scientists investigating DNA repair pathways and genomic stability, CamelBio offers validated antibodies like this anti-XRCC1 polyclonal antibody, alongside optimized monoclonal antibodies, bulk ancillary reagents, and support for rare target raw-material sourcing. Our integrated services help streamline your transition from concept to clinical application.