Anti-XRCC1 Rabbit Monoclonal Antibody for WB, ELISA - P18887

Core Product Specifications and Parameters

Biological Background: XRCC1 Function and Localization

• XRCC1 (X-ray repair cross-complementing protein 1) is a scaffold protein critical for DNA single-strand break repair, mediating the assembly of DNA break repair protein complexes. Related references: PMID:11163244 PMID:28002403
• It negatively regulates ADP-ribosyltransferase activity of PARP1 during base-excision repair to prevent excessive PARP1 activity, binding specifically to auto-poly-ADP-ribosylated PARP1. Related references: PMID:28002403 PMID:34102106 PMID:34811483
• Recognizes and binds poly-ADP-ribose chains, particularly on auto-modified PARP1, limiting its activity. Related references: PMID:14500814 PMID:34102106 PMID:34811483
• The protein localizes to the nucleus and is associated with chromosomes, consistent with its role in DNA repair.
• Expressed in fibroblasts, retinal pigmented epithelial cells, and lymphoblastoid cells at the protein level.
• Undergoes various post-translational modifications including phosphorylation, isopeptide bond formation, and ubiquitination (Ubl conjugation).
• Linked to neurodegeneration when mutated, highlighting its biological significance.

Experimental Guidance and Technical Tips

• The immunogen is located within the N-terminal region (amino acids 1-100); this antibody is likely to detect full-length XRCC1 in applications such as WB and ELISA.
• For optimal performance, use appropriate positive controls, such as human fibroblast lysates or tissues expressing XRCC1, and validate in your specific sample system.
• Cross-reactivity is indicated for human, mouse, and rat, making this antibody suitable for studies across these species; however, independent verification is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your research and development. This anti-XRCC1 antibody supports DNA damage and repair studies, and our team is ready to assist with custom sourcing for your specific diagnostic or laboratory needs.