Anti-XPNPEP3 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NQH7

Core Product Specifications and Parameters

Biological Background: XPNPEP3 Function and Localization

• Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Leu-Pro-Ala. Also shows low activity towards peptides with Ala or Ser at the P1 position. Related references: PMID:25609706 PMID:28476889
• Promotes TNFRSF1B-mediated phosphorylation of MAPK8/JNK1 and MAPK9/JNK2, suggesting an adaptor protein function independent of peptidase activity. May inhibit apoptotic cell death induced via TNF-TNFRSF1B signaling.
• Localizes to the mitochondrion and cytoplasm.
• Widely expressed, with isoform 1 being more abundant than isoform 2.
• Belongs to the M24 metallopeptidase family; requires manganese as a cofactor.
• Contains a mitochondrial transit peptide; associated with nephronophthisis (disease variant).
• Alternative splicing produces multiple isoforms, with isoform 1 representing the canonical sequence.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–250) of human XPNPEP3; confirm epitope conservation in predicted cross-reactive species.
• For Western blot, a primary band near 57kDa is expected, but additional bands may reflect isoforms or post-translational modifications; validate using knockout controls or relevant cell lysates.
• In IF/ICC, the mitochondrial and cytoplasmic localization should be observed; consider co-staining with organelle-specific markers to confirm.
• Cross-reactivity with mouse and rat is predicted; verify compatibility in the intended sample type before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material sourcing partner, offering validated antibody pairs, bulk ancillary reagents, and customized procurement for rare targets. This XPNPEP3 antibody supports research into mitochondrial biology, TNF signaling pathways, and nephronophthisis. For one-stop access from concept to clinic, contact CamelBio to discuss your project requirements.