Anti-XPNPEP3 Polyclonal Antibody (Rabbit) for WB, ELISA - Q9NQH7

Core Product Specifications and Parameters

Biological Background: XPNPEP3 Function and Localization

• XPNPEP3 (Xaa-Pro aminopeptidase 3), also known as Aminopeptidase P3 (APP3), is a manganese-dependent metalloprotease belonging to the M24B family of aminopeptidases. It is encoded by the XPNPEP3 gene and undergoes alternative splicing to generate multiple isoforms.
• The enzyme catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, with preference for Pro, but also shows low activity towards peptides with Ala or Ser at the P1 position. Related references: PMID:25609706 PMID:28476889
• Beyond its catalytic role, XPNPEP3 functions as an adaptor protein in TNF signaling: it promotes TNFRSF1B-mediated phosphorylation of MAPK8/JNK1 and MAPK9/JNK2, independently of its peptidase activity, and may inhibit TNF-induced apoptotic cell death.
• Subcellular localization studies reveal that XPNPEP3 is primarily targeted to the mitochondria and is also found in the cytoplasm; the presence of a mitochondrial transit peptide facilitates its import.
• XPNPEP3 is widely expressed across tissues, with isoform 1 being the more abundant transcript.
• Mutations in XPNPEP3 have been associated with nephronophthisis-like nephropathy (NPHPL1), highlighting its importance in renal function.
• The protein contains a 3D structure and features critical metal-binding residues essential for its catalytic activity.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 32–230 of human XPNPEP3 (NP_071381.1), a region N-terminal to the catalytic domain; thus the antibody may recognize multiple isoforms and proteolytic fragments.
• In Western blotting (WB), multiple bands corresponding to the observed molecular weights (31 kDa, 32 kDa, 48 kDa, 54 kDa, 57 kDa) may be detected; validate the expected bands using positive controls (e.g., human or mouse cell lysates expressing XPNPEP3) and consider the isoform pattern in your sample system.
• For ELISA applications, the polyclonal nature of this antibody can provide robust signal; however, appropriate blocking and washing steps should be optimized, and assay performance should be verified with recombinant protein or native antigen standards.
• Given the antibody’s cross-reactivity with mouse XPNPEP3, it is suitable for studies in mouse models; confirm reactivity in your specific tissue or cell type.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing reliable access to validated antibodies, recombinant proteins, and ancillary reagents. For research targeting XPNPEP3—a mitochondrial peptidase implicated in peptide processing and TNF signaling—CamelBio offers this high-quality rabbit polyclonal antibody alongside a portfolio of complementary products, including matched antibody pairs, human/mouse sample panels, and bulk reagent supply. Our team assists with rare-target raw material procurement and custom antibody development, streamlining the transition from discovery to diagnostic assay manufacturing.