Anti-XPD/ERCC2 Polyclonal Antibody for WB, IF/ICC, ELISA - P18074

Core Product Specifications and Parameters

Biological Background: XPD/ERCC2 Function and Localization

• XPD/ERCC2 is an ATP-dependent 5'-3' DNA helicase and a core component of the transcription/DNA repair factor TFIIH complex, essential for transcription initiation and nucleotide excision repair (NER). Related references: PMID:31253769 PMID:8413672 PMID:9771713
• In NER, XPD/ERCC2 recognizes damaged bases, unwinds DNA around lesions, and facilitates lesion verification and excision of damaged oligonucleotides. Related references: PMID:17466626 PMID:23352696 PMID:31253769
• In transcription, XPD/ERCC2 bridges the CAK kinase module to the core TFIIH complex, enabling promoter opening and RNA polymerase II CTD phosphorylation. Related references: PMID:10024882 PMID:17466626 PMID:33902107
• XPD/ERCC2 localizes primarily to the nucleus, but also associates with the cytoplasm and mitotic spindle, where it participates in chromosome segregation as part of the MMXD complex.
• The protein is also known as ERCC2, BTF2 p80, TFIIH p80, and CXPD; mutations in the ERCC2 gene are linked to Xeroderma pigmentosum, Cockayne syndrome, and trichothiodystrophy.
• XPD/ERCC2 contains iron-sulfur clusters and an ATP-binding domain, and its activity is regulated by post-translational modifications such as ubiquitination.

Experimental Guidance and Technical Tips

• For WB, the predicted molecular weight is approximately 87 kDa; use whole-cell lysates from human or rat cells and consider appropriate loading controls.
• For IF/ICC, validate the antibody in your specific cell/tissue system, as fixation and permeabilization conditions may affect signal.
• As a polyclonal antibody, batch-to-batch consistency should be monitored; pre-adsorption against the immunogen can help confirm specificity.
• This antibody is suitable for ELISA; optimization of coating antigen and detection conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material sourcing partner, providing validated antibodies, bulk ancillary reagents, and custom procurement for rare targets. This anti-XPD/ERCC2 polyclonal antibody supports research in DNA repair, transcription, and the TFIIH complex, and we can assist with complementary reagents such as recombinant proteins or matched antibody pairs. Whether you need one-stop access to core components or tailored sourcing solutions, CamelBio bridges the gap from concept to clinic.