Anti-XPD/ERCC2 Polyclonal Antibody for WB, ELISA - P18074

Core Product Specifications and Parameters

Biological Background: XPD/ERCC2 Function and Localization

• XPD/ERCC2 (ERCC excision repair 2, TFIIH core complex helicase subunit) is an ATP-dependent 5'-3' DNA helicase critical for nucleotide excision repair (NER) and transcription initiation as a component of the TFIIH complex (PubMed:31253769, PubMed:8413672, PubMed:9771713). Related references: PMID:31253769, PMID:8413672, PMID:9771713

• It plays a dual role: in NER, it opens DNA around lesions for damage verification and excision; in transcription, it is essential for promoter opening and escape, bridging the CAK kinase module to the core TFIIH complex (PubMed:10024882, PubMed:17466626, PubMed:23352696, PubMed:33902107). Related references: PMID:10024882, PMID:17466626, PMID:23352696, PMID:33902107

• The protein contains a conserved iron-sulfur (4Fe-4S) cluster essential for its helicase activity and is subject to ubiquitylation (Ubl conjugation) regulating its function.

• XPD/ERCC2 is predominantly nuclear but also localizes to the cytoplasm and mitotic spindle, where it participates in chromosome segregation as part of the MMXD complex.

• Mutations in XPD/ERCC2 are associated with severe genetic disorders including Xeroderma pigmentosum group D, Cockayne syndrome, trichothiodystrophy, and combined phenotypes characterized by UV sensitivity, developmental abnormalities, and premature aging.

• The protein is involved in host-virus interactions and has a putative isomerase activity, reflecting its multifunctional nature in cellular homeostasis.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 1-180 of human XPD/ERCC2, targeting the N-terminal region. This region is well-conserved, supporting cross-reactivity with mouse and rat.
• For Western blotting, a band at approximately 87 kDa is expected (predicted MW 86.9 kDa). Use appropriate loading controls and verify specificity in knockout or knockdown systems.
• ELISA applications may require coating with recombinant antigen or cell lysates; titrate antibody concentrations for optimal signal-to-noise ratio.
• As a polyclonal antibody, lot-to-lot variability is possible. Validate each new batch in the relevant experimental context before large-scale use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated supplier of IVD raw materials, providing diagnostic manufacturers and research laboratories with reliable access to antibodies, antigens, and ancillary reagents. This anti-XPD/ERCC2 polyclonal antibody supports investigations into DNA repair mechanisms, transcription regulation, and associated pathologies such as Xeroderma pigmentosum. Beyond individual products, CamelBio offers comprehensive sourcing solutions—including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk reagents, and support for rare target procurement—streamlining your journey from concept to clinic.