Anti-XIAP Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P98170

Core Product Specifications and Parameters

Biological Background: XIAP Function and Localization

• XIAP is a multi-functional protein that directly inhibits caspases CASP3, CASP7, and CASP9, thereby blocking apoptosis. Related references: PMID:11257230, PMID:11257231, PMID:12620238, PMID:16352606
• As an E3 ubiquitin-protein ligase, XIAP regulates NF-κB signaling and innate immunity by mediating polyubiquitination of RIPK1, RIPK2, and other substrates. Related references: PMID:17560374, PMID:17967870, PMID:19473982, PMID:19667203, PMID:22607974, PMID:29452636
• XIAP contributes to copper homeostasis by ubiquitinating COMMD1 for degradation and enhancing CCS-mediated SOD1 activation. Related references: PMID:14685266, PMID:20154138
• It modulates cell survival pathways including ripoptosome suppression, BCL2 degradation, and Wnt signaling activation via TLE ubiquitination. Related references: PMID:22095281, PMID:29020630, PMID:22304967
• XIAP localizes to both the cytoplasm and nucleus.
• XIAP is ubiquitously expressed, except in peripheral blood leukocytes, and is found in colonic crypts. Related references: PMID:8654366, PMID:30389919

Experimental Guidance and Technical Tips

• For Western blot, consider using whole cell lysates from human, mouse, or rat samples; the expected band is approximately 57 kDa, consistent with the calculated molecular weight.
• In immunofluorescence and immunocytochemistry, optimal fixation and permeabilization should be determined; XIAP signal may be detected in both cytoplasmic and nuclear compartments.
• For ELISA applications, the antibody may be titrated using a recombinant XIAP protein standard, and background may need optimization.
• Cross-reactivity with other IAP family members cannot be excluded due to the polyclonal nature; validation in the relevant sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined, one-stop solution for high-quality IVD raw materials. This anti-XIAP polyclonal antibody supports apoptosis, NF-κB signaling, and ubiquitination research, and is part of our comprehensive portfolio of validated antibodies and recombinant proteins. From optimized monoclonal/polyclonal antibodies and matched antibody pairs to bulk ancillary reagents, CamelBio bridges your raw-material needs from concept to clinic.