Anti-XIAP Rabbit Monoclonal Antibody (KO Validated) for WB, ELISA - P98170

Core Product Specifications and Parameters

Biological Background: XIAP Function and Localization

• XIAP (X-linked inhibitor of apoptosis), also known as BIRC4, IAP3, or hILP, is a member of the inhibitor of apoptosis (IAP) family, characterized by three baculoviral IAP repeat (BIR) domains and a C-terminal RING-type zinc finger domain.
• Serves as a direct and potent caspase inhibitor; it binds the active site pockets of CASP3 and CASP7, obstructing substrate entry, and keeps CASP9 in an inactive monomeric state, thereby blocking apoptosis. Related references: PMID:11257230 PMID:11257231 PMID:12620238 PMID:16352606
• Functions as an E3 ubiquitin-protein ligase, mediating both degradative and non-degradative ubiquitination (including Lys-63-linked chains) of targets such as RIPK1, RIPK2, DIABLO/SMAC, and MAP3K2/MEKK2, thereby modulating NF-kB and MAP kinase signaling pathways. Related references: PMID:17560374 PMID:17967870 PMID:19473982
• Regulates innate immunity by catalyzing Lys-63-linked polyubiquitination of RIPK2 downstream of NOD1 and NOD2, transforming RIPK2 into a scaffolding platform for the LUBAC complex and downstream NF-kB and MAP kinase activation. Related references: PMID:19667203 PMID:22607974 PMID:29452636
• Involved in copper homeostasis via ubiquitination and proteasomal degradation of COMMD1, and enhances the chaperone activity of CCS toward SOD1. Related references: PMID:14685266 PMID:20154138
• Acts as a positive regulator of Wnt signaling by ubiquitinating TLE transcription corepressors (TLE1-4) and AES, which relieves repression on TCF/LEF-mediated transcription and allows beta-catenin/TCF4 complex formation. Related references: PMID:22304967
• Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8, thus protecting cells from caspase-dependent and -independent necroptotic cell death. Related references: PMID:22095281
• Ubiquitously expressed across tissues, but absent from peripheral blood leukocytes; detected in colonic crypts at the protein level. Related references: PMID:8654366 PMID:30389919
• Subcellularly localizes to both the cytoplasm and nucleus, consistent with its diverse functional roles in apoptosis, signaling, and transcription.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 1-200 of human XIAP) covers the N-terminal region containing the BIR domains. This region is critical for XIAP's interaction with caspases and other binding partners; therefore, the antibody may recognize epitopes essential for functional studies.
• For Western blot, a band at approximately 57 kDa is expected based on the predicted molecular weight of XIAP. However, due to potential post-translational modifications (e.g., phosphorylation, ubiquitination) or cleavage products, additional bands may be observed; validate in your sample system.
• This antibody has been reported to react with human, mouse, and rat XIAP by the manufacturer. For cross-species applications (e.g., mouse or rat samples), verify reactivity and specificity under your experimental conditions.
• The antibody is KO validated, indicating a high probability of specificity. Still, include appropriate positive and negative controls (e.g., XIAP knockout lysates) in your assays.
• For ELISA, optimize coating antigen concentration and antibody dilution; the immunogen is a recombinant protein, so consider using a similar recombinant protein as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive supply partner for IVD raw materials, technical services, and consulting, supporting diagnostic manufacturers, research laboratories, and institutes from early-stage development to clinical application. With this XIAP antibody and a broad portfolio of validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents, we help accelerate your research into apoptosis, cancer, and immune signaling pathways. For rare or custom targets, CamelBio offers specialized sourcing capabilities to meet your project’s exact requirements.