Anti-XCR1 Polyclonal Antibody for IHC-P and ELISA - P46094

Core Product Specifications and Parameters

Biological Background: XCR1 Function and Localization

• XCR1, also designated GPR5 and CCXCR1, is a G protein-coupled receptor (GPCR) that selectively binds the XC chemokine XCL1/lymphotactin and XCL2.
• Ligand engagement triggers signal transduction via intracellular calcium mobilization, a hallmark of chemokine receptor activation.
• The receptor localizes to the cell membrane and is predicted to span the membrane seven times, consistent with GPCR topology.
• The XCR1 protein has a calculated molecular weight of approximately 38.5 kDa (based on UniProt entry P46094), and contains a disulfide bond critical for structural integrity.
• As a specific receptor for lymphotactin, XCR1 is thought to participate in directing lymphocyte migration, though tissue-specific expression patterns remain to be fully characterized.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–100) of human XCR1, which is extracellular and may be accessible in native conformation; consider this when designing detection protocols.
• For IHC-P, standard antigen retrieval methods are recommended; validate antibody specificity with positive (XCR1-expressing tissue) and negative (knockout or peptide-blocked) controls.
• In ELISA, this antibody can be used for detection of recombinant XCR1 or XCR1-derived peptides; titration and optimization are advised for each assay format.
• Cross-reactivity with rat XCR1 is noted; if using rat samples, confirm compatibility with the specific tissue or cell line under study.
• The predicted molecular weight is 39 kDa, but post-translational modifications (e.g., glycosylation) may result in band shifts in western blot; note that this antibody is not validated for WB by the supplier.
• As a polyclonal antibody, lot-to-lot variation may occur; always include appropriate controls and standardize conditions between experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, technical services, and consultation, supporting diagnostic manufacturers from early development to clinical application. This anti-XCR1 antibody fits into chemokine receptor and immunological research workflows. Our integrated supply chain offers validated antibody pairs, bulk monoclonal/polyclonal antibodies, and auxiliary reagents to streamline your assay development.