Anti-XCP1 Rabbit Polyclonal Antibody for WB and ELISA - B6TIC7

Core Product Specifications and Parameters

Biological Background: XCP1 Function and Localization

• XCP1, also known as cysteine protease 5, is a maize cysteine protease belonging to the papain family of proteolytic enzymes.
• It is predicted to function as a hydrolase and protease, likely involved in protein turnover, maturation, or defense responses.
• Subcellular localization studies indicate that XCP1 is associated with the cell membrane and vacuole, consistent with roles in both intracellular and extracellular proteolysis.
• The protein contains a signal peptide, suggesting it is targeted to the secretory pathway.
• XCP1 is identified as a glycoprotein, which may contribute to its stability, trafficking, or substrate recognition.
• The presence of disulfide bonds is typical for cysteine proteases and likely important for maintaining the correct three-dimensional structure in the oxidizing environments of the vacuole or extracellular space.
• Keywords such as "Proteomics identification" indicate that XCP1 has been detected in proteomic studies, supporting its expression in maize tissues.

Experimental Guidance and Technical Tips

• For Western blot, a band around 71 kDa is expected; however, due to glycosylation, the apparent molecular weight may differ from the predicted 41 kDa.
• The antibody may recognize both precursor and mature forms of XCP1.
• Include appropriate positive controls (e.g., maize tissue lysates known to express XCP1).
• For ELISA, consider using the recombinant immunogen as a standard.
• Validate antibody specificity in relevant sample systems (e.g., maize cultivars) using blocking peptides or knockout lines if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated antibodies, optimized monoclonal/polyclonal antibody pairs, bulk ancillary reagents, and rare target sourcing—ideal for researchers studying maize cysteine proteases or developing plant-based diagnostic assays. Whether you need this anti-XCP1 antibody or custom solutions for your entire workflow, CamelBio bridges the gap from concept to clinic.