Anti-XBP-1s Rabbit Monoclonal Antibody for WB, ChIP, ELISA - P17861-2

Core Product Specifications and Parameters

Biological Background: XBP-1s Function and Localization

• XBP-1s is the active, spliced isoform of X-box-binding protein 1 (XBP1), also named Tax-responsive element-binding protein 5 (TREB-5). It acts as a stress-inducible transcriptional activator central to the unfolded protein response (UPR) during ER stress. Related references: PMID:11779464, PMID:25239945
• It upregulates ER chaperones and ER-associated degradation (ERAD) components to preserve ER proteostasis (PubMed:11779464, PubMed:25239945). Related references: PMID:11779464, PMID:25239945
• XBP-1s drives phospholipid biosynthesis and ER membrane expansion (PubMed:15466483). Related references: PMID:15466483
• It promotes VEGF-dependent endothelial cell proliferation and angiogenesis via β-catenin/E2F2 (PubMed:23529610), and triggers caspase-dependent apoptosis linked to atherosclerosis (PubMed:19416856, PubMed:23529610). Related references: PMID:23529610, PMID:19416856
• XBP-1s mediates endostatin-induced autophagy through BECN1 transcriptional activation (PubMed:23184933). Related references: PMID:23184933
• As an oncogenic factor, it induces SNAI1 to promote epithelial-mesenchymal transition and breast cancer cell invasion (PubMed:25280941). Related references: PMID:25280941
• The protein localizes to both nucleus and cytoplasm and binds DNA motifs such as ERSE, UPRE, and 5′-CCACG-3′ to regulate metabolic, inflammatory, and differentiation programs.

Experimental Guidance and Technical Tips

• The immunogen corresponds to recombinant human XBP-1s residues 188–287; the antibody is expected to recognize the spliced active isoform.
• For Western blotting under reducing conditions, a band near 40 kDa is predicted based on the protein’s molecular weight; validate in the relevant sample system under ER stress induction (e.g., tunicamycin treatment).
• In ChIP assays, XBP-1s binds to ERSE and related elements; include positive control loci (e.g., BiP/GRP78 promoter) and confirm enrichment by qPCR.
• For ELISA, pair with a detection antibody recognizing a distinct epitope; titrate both antibodies using recombinant XBP-1s protein as standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD development by providing validated monoclonal antibodies like this anti-XBP-1s clone, optimized for WB, ChIP, and ELISA. For ER stress and UPR research, we also supply antibody pairs, bulk ancillary reagents, and can source rare-target raw materials to support your assay development from concept to clinic.