Anti-Xanthine Oxidase (XDH) Rabbit Polyclonal Antibody for WB and ELISA - P47989

Core Product Specifications and Parameters

Biological Background: Xanthine Oxidase (XDH) Function and Localization

• Xanthine dehydrogenase/oxidase (XDH, also known as XO, XOR, XAN1) is a key enzyme in purine degradation.
• Catalyzes the oxidation of hypoxanthine to xanthine and xanthine to uric acid, crucial steps in purine catabolism.
• Contributes to generation of reactive oxygen species; also exhibits low oxidase activity toward aldehydes in vitro.
• Contains cofactors: 2Fe-2S clusters, FAD, molybdenum, NAD, indicating complex redox activity (Keywords: 2Fe-2S, FAD, Flavoprotein, Iron-sulfur, Molybdenum, NAD, Oxidoreductase).
• Localized to cytoplasm and peroxisomes; also secreted, and detected in milk at protein level.
• Post-translational modifications include disulfide bonds (Keywords: Disulfide bond). Relevant disease variants exist (Keywords: Disease variant).
• Calculated molecular weight: 146424 Da.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 985-1084 of human XDH; consider this region for epitope recognition and sequence conservation across species.
• For Western blot, the predicted protein weight is ~146 kDa; ensure appropriate gel percentage and transfer conditions.
• The peptide-based design may enable detection in both reducing/denaturing and native conditions; validate in the relevant sample system.
• Cross-reactivity with mouse and rat is reported; verify specificity in your experimental model.
• For ELISA, the antibody may be suitable as a capture or detection reagent; optimize coating and blocking conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our Xanthine Oxidase (XDH) antibody supports research in purine metabolism and oxidative stress. We offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your development workflow.