Anti-Xanthine Oxidase (XDH) Rabbit Monoclonal Antibody for WB - P47989

Core Product Specifications and Parameters

Biological Background: Xanthine Oxidase (XDH) Function and Localization

• Xanthine dehydrogenase/oxidase (XDH, also known as XO or XOR) is a key enzyme in purine catabolism, catalyzing the oxidation of hypoxanthine to xanthine and xanthine to uric acid.
• During these reactions, the enzyme contributes to the generation of reactive oxygen species (ROS), playing a role in oxidative stress-related processes.
• In vitro, XDH exhibits low oxidase activity towards aldehydes, indicating broader substrate specificity under certain conditions.
• The enzyme contains several cofactors essential for its activity: two 2Fe-2S clusters, FAD, and a molybdenum cofactor (molybdopterin).
• Subcellularly, XDH localizes to the cytoplasm and peroxisomes, and has been detected as a secreted protein in milk.
• Tissue-specific expression is confirmed in human milk at the protein level, though its expression in other tissues is inferred at the mRNA level.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 202-293 of human XDH, a region that shares high homology with mouse and rat XDH, which may explain the reported cross-reactivity.
• For Western blotting, expect a band at approximately 146 kDa under reducing conditions; consider using fresh protease inhibitors to avoid degradation.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), the antibody may detect cytoplasmic and peroxisomal staining patterns; antigen retrieval optimization is recommended.
• For ELISA applications, pairing with a suitable detection antibody may enhance sensitivity; consider testing in a sandwich ELISA format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with consolidated access to premium IVD raw materials, including validated monoclonal antibodies for metabolism-related targets like XDH. We offer comprehensive support across the development pipeline—from bulk antibody pairs and ancillary reagents to specialized custom sourcing for rare targets. Our integrated supply model helps streamline your journey from concept to clinic.