Anti-Xanthine Dehydrogenase/Oxidase (XDH) Monoclonal Antibody for WB, IHC-P, ELISA - P47989

Core Product Specifications and Parameters

Biological Background: Xanthine Dehydrogenase/Oxidase Function and Localization

• Xanthine dehydrogenase/oxidase (XDH, also known as XO, XOR, XAN1) is a key enzyme in purine degradation, catalyzing the sequential oxidation of hypoxanthine to xanthine and xanthine to uric acid.
• Contributes to the generation of reactive oxygen species (ROS) during oxidative reactions, which may play roles in signaling and oxidative stress.
• Exhibits low oxidase activity towards aldehydes in vitro.
• Subcellular localization includes cytoplasm, peroxisomes, and a secreted form; expression is detected in milk at the protein level.
• Contains 2Fe-2S clusters, FAD, a molybdenum cofactor, and an NAD-binding domain, classifying it as a complex oxidoreductase.
• Genetic mutations in XDH are associated with xanthinuria type I, a disorder of purine metabolism.
• The protein has a predicted molecular weight of approximately 146 kDa, consistent with the observed product weight.

Experimental Guidance and Technical Tips

• The immunogen targets a C-terminal region (aa 1000–1100) that is likely conserved between dehydrogenase and oxidase forms; epitope accessibility may be influenced by sample preparation.
• For Western blot, consider using positive control lysates from human liver or milk, and validate the observed ~146 kDa band.
• In IHC-P, optimization of antigen retrieval is recommended; expect cytoplasmic and peroxisomal staining patterns depending on tissue context.
• For ELISA formats, confirm specificity with recombinant XDH protein or native enzyme under standard assay conditions.
• Given reported cross-reactivity with mouse and rat, verify detection in the relevant species and tissue types to ensure suitability for your model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs to premium IVD raw materials like this anti-Xanthine Dehydrogenase/Oxidase monoclonal antibody, supporting purine metabolism and oxidative stress investigations. Beyond individual antibodies, we offer validated antibody pairs, optimized monoclonal and polyclonal reagents, and bulk ancillary materials, along with sourcing for rare targets—streamlining development from concept to clinic.