Anti-WWP2 Rabbit Polyclonal Antibody for WB and ELISA - O00308

Core Product Specifications and Parameters

Biological Background: WWP2 Function and Localization

• WWP2, also known as NEDD4-like E3 ubiquitin-protein ligase WWP2, Atrophin-1-interacting protein 2 (AIP2), or WW domain-containing protein 2, is a member of the HECT-type E3 ubiquitin ligase family. The gene undergoes alternative splicing, generating multiple isoforms.
• Functions as an E3 ubiquitin ligase, accepting ubiquitin from an E2 enzyme and transferring it to specific substrates. It catalyzes polyubiquitination via Lys-63 linkages, predominantly targeting substrates for proteasomal degradation.
• In embryonic stem cells, WWP2 ubiquitinates and promotes degradation of the pluripotency factor POU5F1 (OCT4), thereby contributing to the regulation of stem cell maintenance and differentiation.
• Also ubiquitinates EGR2 in T-cells, leading to its proteasomal degradation and inhibition of activation-induced cell death, highlighting a role in immune regulation.
• Localizes primarily to the nucleus, consistent with its function in regulating nuclear transcription factors and chromatin-associated proteins.
• Detected across a broad range of tissues including heart, brain, placenta, lung, liver, muscle, kidney, pancreas, spleen, and peripheral blood leukocytes.
• Classified as a phosphoprotein and involved in host-virus interactions, underscoring its regulatory complexity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the internal region (aa 151-400) of human WWP2, encompassing part of the catalytic HECT domain. This design may facilitate detection of both full-length and certain truncated or alternatively spliced isoforms in Western blot.
• For Western blot analysis, optimize antibody dilution (e.g., 1:500–1:2000) and incubation conditions using appropriate positive control lysates (e.g., human cell lines expressing WWP2).
• Since this is a polyclonal antibody, batch-to-batch variability may exist; include appropriate controls such as recombinant protein or siRNA knockdown/knockout lysates to confirm specificity.
• In ELISA applications, consider using the recombinant immunogen or purified protein as a coating antigen, and titrate the antibody to establish optimal signal-to-noise ratios.
• Cross-reactivity is confirmed for human; reactivity with other species has not been tested and should be validated if required for your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines your IVD development by supplying validated raw materials for ubiquitination and protein degradation research. From this anti-WWP2 polyclonal antibody and matched antigen pairs to optimized ancillary reagents, we support every stage from research to manufacturing. Our one-stop sourcing model accelerates diagnostic kit development for E3 ligase targets, helping you move from concept to clinic with confidence.