Anti-WWOX Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NZC7

Core Product Specifications and Parameters

Biological Background: WWOX Function and Localization

• WWOX (WW domain-containing oxidoreductase) is a putative oxidoreductase and established tumor suppressor.
• It plays a critical role in apoptosis, is required for normal bone development (by similarity), and may act synergistically with p53/TP53 to mediate genotoxic stress-induced cell death.
• WWOX participates in TGFB1 signaling and TGFB1-mediated cell death, and also contributes to tumor necrosis factor (TNF)-induced cell death.
• The protein inhibits Wnt signaling, likely by sequestering DVL2 in the cytoplasm.
• Subcellular localization is versatile, including the cytoplasm, nucleus, mitochondrion, Golgi apparatus, and lysosome.
• It is widely expressed, with strong expression in testis, prostate, and ovary; certain isoforms (5 and 6) may be restricted to tumor cell lines.
• Key post-translational features include phosphorylation and ubiquitination, and the protein belongs to the short-chain dehydrogenase/reductase family.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N‑terminal region (amino acids 1–120) of human WWOX, which may detect full‑length and some N‑terminal isoforms.
• For western blot, anticipate the major band near 47kDa; alternatively spliced or modified forms may appear at different sizes, so validate in the relevant sample system.
• Subcellular fractionation or immunofluorescence controls should account for the protein’s multi‑organelle distribution; colocalization markers may enhance interpretation.
• In ELISA, consider using recombinant WWOX or lysates from cell lines with known expression levels as reference standards.
• Optimal working dilutions should be determined empirically, and cross‑reactivity with mouse enables translational studies but should be confirmed in the intended model.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with efficient one‑stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For research into tumor suppression, apoptosis, or developmental pathways, we can streamline procurement of the anti‑WWOX antibody and other rare‑target reagents, supporting your projects from concept to clinic. Our supply capabilities help minimize sourcing complexity while maintaining the reliability required for immunoassay development and validation.