Anti-WWOX Polyclonal Antibody for WB, ELISA - Q9NZC7

Core Product Specifications and Parameters

Biological Background: WWOX Function and Localization

• Protein Identity and Isoforms: WWOX encodes the WW domain-containing oxidoreductase, also known as FOR, WOX1, or SDR41C1. Alternative splicing produces multiple isoforms; isoform 5 and isoform 6 may be restricted to tumor cell lines.
• Tumor Suppressor and Apoptosis: Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development (by similarity). May function synergistically with p53/TP53 to control genotoxic stress-induced cell death.
• TGFB1 and TNF Signaling: Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also participate in tumor necrosis factor (TNF)-mediated cell death.
• Wnt Pathway Inhibition: Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm.
• Subcellular Localization: Localizes to the cytoplasm, nucleus, mitochondrion, Golgi apparatus, and lysosome.
• Tissue Expression: Widely expressed, with strong expression in testis, prostate, and ovary. Overexpressed in some cancer cell lines.
• Post-Translational Modifications and Keywords: Annotated as a phosphoprotein and subject to ubiquitin conjugation. Keywords include alternative splicing, disease variant (epilepsy, neurodegeneration), and NADP binding.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-120) of human WWOX, which may detect full-length and some N-terminal isoforms. For Western blot, consider a validated positive control lysate from a cell line with endogenous expression.
• As a rabbit polyclonal antibody, the product exhibits broad reactivity (human, mouse, rat). Optimize the dilution factor for each application; typical starting dilutions for WB are 1:500-1:2000.
• For ELISA, pair this polyclonal with a monoclonal capture antibody if developing a sandwich assay. Validate the detection system in the relevant sample matrix.

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